在一项体内研究中,Cladophora glomerata Kützing 提取物具有抗氧化、抗炎和抗亚硝基压力的作用,可防止肾脏有机阴离子转运受损。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2024-10-01 Epub Date: 2024-07-19 DOI:10.4162/nrp.2024.18.5.633
Atcharaporn Ontawong, Chaliya J Aida, Pornpun Vivithanaporn, Doungporn Amornlerdpison, Chutima S Vaddhanaphuti
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引用次数: 0

摘要

背景/目的:据报道,富含多酚的肾小球衣藻提取物(CGE)通过调节蛋白激酶介导的有机阴离子转运体 1(Oat1)和 3(Oat3)的功能,对 2 型糖尿病(T2DM)大鼠具有抗糖尿病和肾保护作用。然而,CGE 对肾脏保护的抗氧化作用尚未得到研究。本研究在一项体内研究中考察了 CGE 对肾脏有机阴离子转运功能的抗氧化作用的相关机制:材料/方法:通过高脂饮食结合单剂量 40 毫克/千克体重(BW)链脲佐菌素诱导大鼠患糖尿病。随后,给正常饮食大鼠补充载体或 1,000 毫克/千克体重的 CGE,给 T2DM 大鼠补充载体、CGE 或 200 毫克/千克体重的维生素 C,持续 12 周。该研究评估了 T2DM 的一般特征和肾氧化应激指标。在正常饮食组(ND)和 ND + CGE 治疗组中,通过使用肾皮质切片测量对氨基酚(PAH)摄取量和肾脏炎症细胞因子表达,评估了肾脏的有机物转运功能:结果:补充 CGE 能明显降低 T2DM 大鼠的高血糖、高甘油三酯血症、胰岛素抵抗和肾脏脂质过氧化反应。CGE 的抗肾脏炎症作用体现在降低肿瘤坏死因子-1α 和白细胞介素-1β。特别值得注意的是,CGE 直接减弱了硝普钠诱导的肾脏氧化/亚硝基应激,并介导了正常处理的 CGE 大鼠对多环芳烃的吸收。这些数据还与一氧化氮产生的减少有关,凸显了 CGE 作为治疗剂治疗 T2DM 相关肾脏并发症的潜力:这些研究结果表明,CGE 具有抗糖尿病作用,可通过氧化/亚硝基应激途径直接预防糖尿病肾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cladophora glomerata Kützing extract exhibits antioxidant, anti-inflammation, and anti-nitrosative stress against impairment of renal organic anion transport in an in vivo study.

Background/objectives: Cladophora glomerata extract (CGE), rich in polyphenols, was reported to exhibit antidiabetic and renoprotective effects by modulating the functions of protein kinases-mediated organic anion transporter 1 (Oat1) and 3 (Oat3) in rats with type 2 diabetes mellitus (T2DM). Nevertheless, the antioxidant effects of CGE on such renoprotection have not been investigated. This study examined the mechanisms involved in the antioxidant effects of CGE on renal organic anion transport function in an in vivo study.

Materials/methods: Diabetes was induced in the rats through a high-fat diet combined with a single dose of 40 mg/kg body weight (BW) streptozotocin. Subsequently, normal-diet rats were supplemented with a vehicle or 1,000 mg/kg BW of CGE, while T2DM rats were supplemented with a vehicle, CGE, or 200 mg/kg BW of vitamin C for 12 weeks. The study evaluated the general characteristics of T2DM and renal oxidative stress markers. The renal organic transport function was assessed by measuring the para-aminohippurate (PAH) uptake using renal cortical slices and renal inflammatory cytokine expression in the normal diet (ND) and ND + CGE treated groups.

Results: CGE supplementation significantly reduced hyperglycemia, hypertriglyceridemia, insulin resistance, and renal lipid peroxidation in T2DM rats. This was accompanied by the normalization of high expressions of renal glutathione peroxidase and nuclear factor kappa B by CGE and vitamin C. The renal anti-inflammation of CGE was evidenced by the reduction of tumor necrosis factor-1α and interleukin-1β. CGE directly blunted sodium nitroprusside-induced renal oxidative/nitrosative stresses and mediated the PAH uptake in the normally treated CGE in rats was particularly noteworthy. These data also correlated with reduced nitric oxide production, highlighting the potential of CGE as a therapeutic agent for managing T2DM-related renal complications.

Conclusion: These findings suggest that CGE has antidiabetic effects and directly prevents diabetic nephropathy through oxidative/nitrosative stress pathways.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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