一系列三尖杉菌效应物抑制植物免疫反应。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Elisha Thynne, Haider Ali, Kyungyong Seong, Mohammad Abukhalaf, Marco A Guerreiro, Victor M Flores-Nunez, Rune Hansen, Ana Bergues, Maja J Salman, Jason J Rudd, Kostya Kanyuka, Andreas Tholey, Ksenia V Krasileva, Graeme J Kettles, Eva H Stukenbrock
{"title":"一系列三尖杉菌效应物抑制植物免疫反应。","authors":"Elisha Thynne, Haider Ali, Kyungyong Seong, Mohammad Abukhalaf, Marco A Guerreiro, Victor M Flores-Nunez, Rune Hansen, Ana Bergues, Maja J Salman, Jason J Rudd, Kostya Kanyuka, Andreas Tholey, Ksenia V Krasileva, Graeme J Kettles, Eva H Stukenbrock","doi":"10.1111/mpp.13500","DOIUrl":null,"url":null,"abstract":"<p><p>Zymoseptoria tritici is the most economically significant fungal pathogen of wheat in Europe. However, despite the importance of this pathogen, the molecular interactions between pathogen and host during infection are not well understood. Herein, we describe the use of two libraries of cloned Z. tritici effectors that were screened to identify effector candidates with putative pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI)-suppressing activity. The effectors from each library were transiently expressed in Nicotiana benthamiana, and expressing leaves were treated with bacterial or fungal PAMPs to assess the effectors' ability to suppress reactive oxygen species (ROS) production. From these screens, numerous effectors were identified with PTI-suppressing activity. In addition, some effectors were able to suppress cell death responses induced by other Z. tritici secreted proteins. We used structural prediction tools to predict the putative structures of all of the Z. tritici effectors and used these predictions to examine whether there was enrichment of specific structural signatures among the PTI-suppressing effectors. From among the libraries, multiple members of the killer protein-like 4 (KP4) and killer protein-like 6 (KP6) effector families were identified as PTI suppressors. This observation is intriguing, as these protein families were previously associated with antimicrobial activity rather than virulence or host manipulation. This data provides mechanistic insight into immune suppression by Z. tritici during infection and suggests that, similar to biotrophic pathogens, this fungus relies on a battery of secreted effectors to suppress host immunity during early phases of colonization.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 10","pages":"e13500"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470090/pdf/","citationCount":"0","resultStr":"{\"title\":\"An array of Zymoseptoria tritici effectors suppress plant immune responses.\",\"authors\":\"Elisha Thynne, Haider Ali, Kyungyong Seong, Mohammad Abukhalaf, Marco A Guerreiro, Victor M Flores-Nunez, Rune Hansen, Ana Bergues, Maja J Salman, Jason J Rudd, Kostya Kanyuka, Andreas Tholey, Ksenia V Krasileva, Graeme J Kettles, Eva H Stukenbrock\",\"doi\":\"10.1111/mpp.13500\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Zymoseptoria tritici is the most economically significant fungal pathogen of wheat in Europe. However, despite the importance of this pathogen, the molecular interactions between pathogen and host during infection are not well understood. Herein, we describe the use of two libraries of cloned Z. tritici effectors that were screened to identify effector candidates with putative pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI)-suppressing activity. The effectors from each library were transiently expressed in Nicotiana benthamiana, and expressing leaves were treated with bacterial or fungal PAMPs to assess the effectors' ability to suppress reactive oxygen species (ROS) production. From these screens, numerous effectors were identified with PTI-suppressing activity. In addition, some effectors were able to suppress cell death responses induced by other Z. tritici secreted proteins. We used structural prediction tools to predict the putative structures of all of the Z. tritici effectors and used these predictions to examine whether there was enrichment of specific structural signatures among the PTI-suppressing effectors. From among the libraries, multiple members of the killer protein-like 4 (KP4) and killer protein-like 6 (KP6) effector families were identified as PTI suppressors. This observation is intriguing, as these protein families were previously associated with antimicrobial activity rather than virulence or host manipulation. This data provides mechanistic insight into immune suppression by Z. tritici during infection and suggests that, similar to biotrophic pathogens, this fungus relies on a battery of secreted effectors to suppress host immunity during early phases of colonization.</p>\",\"PeriodicalId\":18763,\"journal\":{\"name\":\"Molecular plant pathology\",\"volume\":\"25 10\",\"pages\":\"e13500\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470090/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular plant pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/mpp.13500\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.13500","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

三尖杉属真菌(Zymoseptoria tritici)是欧洲小麦上最具经济意义的真菌病原体。然而,尽管这种病原体非常重要,但人们对其感染过程中病原体与宿主之间的分子相互作用却不甚了解。在本文中,我们介绍了利用两个克隆的 Z. tritici 效应子文库进行筛选,以确定具有假定病原体相关分子模式(PAMP)触发免疫(PTI)抑制活性的候选效应子。每个文库中的效应物在烟草中瞬时表达,用细菌或真菌的 PAMPs 处理表达叶片,以评估效应物抑制活性氧(ROS)产生的能力。通过这些筛选,发现了许多具有 PTI 抑制活性的效应物。此外,一些效应物还能抑制其他三尖杉属真菌分泌蛋白诱导的细胞死亡反应。我们使用结构预测工具预测了所有 Z. tritici 效应子的推定结构,并利用这些预测结果研究了 PTI 抑制效应子是否富集了特定的结构特征。在这些库中,类杀伤蛋白 4(KP4)和类杀伤蛋白 6(KP6)效应子家族的多个成员被鉴定为 PTI 抑制因子。这一观察结果耐人寻味,因为这些蛋白家族以前与抗微生物活性而不是毒力或宿主操纵有关。这些数据从机理上揭示了三尖杉属真菌在感染期间的免疫抑制作用,并表明这种真菌与生物营养型病原体类似,在定殖的早期阶段依靠一系列分泌效应子抑制宿主免疫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An array of Zymoseptoria tritici effectors suppress plant immune responses.

Zymoseptoria tritici is the most economically significant fungal pathogen of wheat in Europe. However, despite the importance of this pathogen, the molecular interactions between pathogen and host during infection are not well understood. Herein, we describe the use of two libraries of cloned Z. tritici effectors that were screened to identify effector candidates with putative pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI)-suppressing activity. The effectors from each library were transiently expressed in Nicotiana benthamiana, and expressing leaves were treated with bacterial or fungal PAMPs to assess the effectors' ability to suppress reactive oxygen species (ROS) production. From these screens, numerous effectors were identified with PTI-suppressing activity. In addition, some effectors were able to suppress cell death responses induced by other Z. tritici secreted proteins. We used structural prediction tools to predict the putative structures of all of the Z. tritici effectors and used these predictions to examine whether there was enrichment of specific structural signatures among the PTI-suppressing effectors. From among the libraries, multiple members of the killer protein-like 4 (KP4) and killer protein-like 6 (KP6) effector families were identified as PTI suppressors. This observation is intriguing, as these protein families were previously associated with antimicrobial activity rather than virulence or host manipulation. This data provides mechanistic insight into immune suppression by Z. tritici during infection and suggests that, similar to biotrophic pathogens, this fungus relies on a battery of secreted effectors to suppress host immunity during early phases of colonization.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信