镉中毒的细胞学效应和槲皮素的保护作用:基于睾丸固有层的机制探索

IF 2.9 4区 工程技术 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Dian Wang, Yi Xiang, Zhaoxuan Zhu, Jiyue Liu, Yisheng Wang, Zeyu Xu, Si Chen, Chunyuan Dai, Jiasen Feng, Jie Chen, Qianhui Ma, Ping Yang
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引用次数: 0

摘要

这项综合研究深入探讨了有毒重金属镉(Cd)对睾丸固有层(LP)的有害影响,LP是精子发生和维持睾丸干细胞龛位的关键角色。研究利用透射电子显微镜、免疫组织化学和双标记免疫荧光技术,分析了镉暴露下小鼠睾丸固有层的结构和细胞成分,并研究了槲皮素的保护作用。研究结果表明,镉暴露会导致睾丸LP的形态和细胞发生显著变化,包括管周肌细胞凋亡、具有抗凋亡行为的CD34+基质细胞激增,以及I型胶原纤维和细胞外基质的过度生成。值得注意的是,槲皮素通过逆转细胞凋亡、减少CD34+基质细胞增殖和处理纤维化标志物,有效抵消了这些不利变化,从而减轻了镉对细胞的损伤。这项研究不仅强调了细胞凋亡和纤维化在镉相关睾丸损伤中的关键影响,还阐明了槲皮素的保护机制,为今后通过细胞疗法和药物干预解决重金属中毒引起的睾丸损伤的临床应用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytological Effects of Cadmium Poisoning and the Protective Effect of Quercetin: A Mechanism Exploration based on the Testicular Lamina Propria.

This comprehensive study delved into the detrimental effects of cadmium (Cd), a toxic heavy metal, on the testicular lamina propria (LP), a key player in spermatogenesis, and the maintenance of testicular stem cell niches. Utilizing transmission electron microscopy, immunohistochemistry, and double-labeling immunofluorescence, the research characterized the structural and cellular components of mouse testicular LP under Cd exposure and investigated the protective effects of quercetin. The findings illustrated that Cd exposure results in significant morphological and cellular modifications within the LP, including the apoptosis of peritubular myoid cells, an upsurge in CD34+ stromal cells displaying anti-apoptotic behaviors, and an excessive production of collagen Type I fibers and extracellular matrix. Remarkably, quercetin effectively counteracted these adverse changes by reversing apoptosis, reducing the proliferation of CD34+ stromal cells, and addressing fibrosis markers, thereby mitigating the cellular damage induced by Cd. This study not only highlighted the critical impact of apoptosis and fibrosis in Cd-related testicular damage but also elucidated the protective mechanism of quercetin, laying the groundwork for future clinical applications in addressing testicular damage from heavy metal poisoning through cellular therapeutics and pharmacological interventions.

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来源期刊
Microscopy and Microanalysis
Microscopy and Microanalysis 工程技术-材料科学:综合
CiteScore
1.10
自引率
10.70%
发文量
1391
审稿时长
6 months
期刊介绍: Microscopy and Microanalysis publishes original research papers in the fields of microscopy, imaging, and compositional analysis. This distinguished international forum is intended for microscopists in both biology and materials science. The journal provides significant articles that describe new and existing techniques and instrumentation, as well as the applications of these to the imaging and analysis of microstructure. Microscopy and Microanalysis also includes review articles, letters to the editor, and book reviews.
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