用于软组织修复的可注射肽-氨基葡聚糖水凝胶:用于细胞核增大的体外评估。

IF 5.2 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
James P. Warren, Ruth H. Coe, Matthew P. Culbert, Andrew R. Dixon, Danielle E. Miles, Marlène Mengoni, Paul A. Beales and Ruth K. Wilcox
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引用次数: 0

摘要

我们报告了肽-糖胺聚糖水凝胶作为可注射生物材料用于承重软组织修复的研发情况。这种水凝胶可作为液体注射用于临床,能在原位快速形成凝胶,并能模拟天然组织的渗透膨胀行为。我们使用了一种新的体外模型来证明水凝胶作为椎间盘退变治疗的髓核增强材料的应用。我们的研究将复杂的实验室凝胶制备方法与简单的临床台式工艺进行了比较。结果表明,pH 值的差异对凝胶的形成没有明显影响,温度的变化对凝胶的性能也没有影响。流变学结果表明,台式混合或针头注射后的凝胶具有一致性。在我们的体外椎间盘退变模型中,我们证实多肽增量可以恢复原生生物力学特性。这表明微创多肽-GAG凝胶递送是可行的,它能在体外恢复椎间盘高度和硬度的同时,在不同温度和针头大小下保持稳定的性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Injectable peptide-glycosaminoglycan hydrogels for soft tissue repair: in vitro assessment for nucleus augmentation†

Injectable peptide-glycosaminoglycan hydrogels for soft tissue repair: in vitro assessment for nucleus augmentation†

We report the development of peptide-glycosaminoglycan hydrogels as injectable biomaterials for load-bearing soft tissue repair. The hydrogels are injectable as a liquid for clinical delivery, rapidly form a gel in situ, and mimic the osmotic swelling behaviour of natural tissue. We used a new in vitro model to demonstrate their application as a nucleus augmentation material for the treatment of intervertebral disc degeneration. Our study compared a complex lab gel preparation method to a simple clinical benchtop process. We showed pH differences did not significantly affect gel formation, and temperature variations had no impact on gel performance. Rheological results demonstrated consistency after benchtop mixing or needle injection. In our in vitro disc degeneration model, we established that peptide augmentation could restore the native biomechanical properties. This suggests the feasibility of minimally invasive peptide-GAG gel delivery, maintaining consistent properties across temperature and needle sizes while restoring disc height and stiffness in vitro.

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来源期刊
Materials Advances
Materials Advances MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
7.60
自引率
2.00%
发文量
665
审稿时长
5 weeks
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