利用光生物调制技术开发抑菌中心静脉端口:体外对比研究。

IF 2.1 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Yuki Takara, Daisuke Yunaiyama, Mika Yasutomi, Tsunenori Arai, Kazutaka Nara, Shigeki Nakamura, Toru Saguchi, Motoki Nakai, Kazuhiro Saito
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引用次数: 0

摘要

当细胞暴露在低能量强度下时,就会发生光生物调制(PBM)。一种新型的中心静脉端口(CVP)已经建立,该端口装有发光二极管(LED),可通过无线能量传输技术发出波长为 680 纳米的红光。这项体外对比研究考察了 PBM 是否能在体外减少耐甲氧西林金黄色葡萄球菌(MRSA)的生长,MRSA 是导致中心静脉(CV)感染的常见原因。在这项体外比较研究中,使用波长为 680 纳米的红光对磷酸盐缓冲盐水中的 MRSA 悬浮液进行照射,照射时间为 7.5、15、30 或 60 分钟,照射容器为 3.5 厘米、面积为 8.5 平方厘米的培养皿。7.5 分钟时的总能量为 85 J,15 分钟时为 170 J,30 分钟时为 340 J,60 分钟时为 680 J。每个时间段准备了 6 个培养皿和 6 个温控样品。每个样品在 37℃下培养过夜。采用 Shapiro-wilk 检验确定数据是否呈正态分布。菌落数计数采用单因素方差分析和 Bonferroni 事后检验进行比较。对照组的平均菌落数为 60.3 个,而辐照组的菌落数在 7.5 分钟时为 51.4 个,15 分钟时为 53.5 个,30 分钟时为 44.6 个,60 分钟时为 34.3 个。60 分钟辐照组的平均菌落数与对照组、7.5 分钟组和 15 分钟组的平均菌落数有显著差异。Bonferroni 的事后检验表明,对照组与 30 分钟对照组与 60 分钟对照组、7.5 分钟对照组与 60 分钟对照组、15 分钟对照组与 60 分钟对照组之间的菌落数差异显著。用 680 纳米 LED 对 MRSA 进行 30 分钟 340 J 和 60 分钟 680 J 的光生物处理可抑制细胞菌落的生长。这些研究结果支持在中心静脉端口使用光生物调节技术来预防中心静脉端口-血流感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of bacteriostatic central venous port using photobiomodulation: a comparative in vitro study.

Photobiomodulation (PBM) occurs when a cell is exposed to low energy intensities. A novel central venous port (CVP) with light-emitting diodes (LEDs) that emits red light with a wavelength of 680 nm via wireless energy transmission technology has been established. This comparative in vitro study examined whether PBM can reduce the growth of methicillin-resistant Staphylococcus aureus (MRSA), a common cause of central venous (CV) infections, in vitro. In this comparative in vitro study, the red light with a wavelength of 680 nm was used to irradiate an MRSA suspension in phosphate-buffered saline for 7.5, 15, 30, or 60 min in a 3.5 cm Petri dish with an area of 8.5 cm2. The total energy was 85 J at 7.5 min, 170 J at 15 min, 340 J at 30 min, and 680 J at 60 min. Six dishes for each time and 6 temperature-controlled samples were prepared. Each sample was incubated overnight at 37℃. The Shapiro-wilk test was used to determine whether the data were normally distributed. The numbers of colonies were counted and compared using one-factor ANOVA and Bonferroni's post-hoc test. The mean numbers of colonies in the control group were 60.3, where the numbers of colonies in the irradiated group were 51.4 at 7.5 min, 53.5 at 15 min, 44.6 at 30 min, 34.3 at 60 min. The mean number of colonies in the 60 min irradiated group differed significantly from that in the control, 7.5 min, and 15 min groups. The Bonferroni's post-hoc test showed significant difference in the number of colonies between control vs. 30 min control vs. 60 min, 7.5 min vs. 60 min, 15 min vs. 60 min. PBM with 680 nm LEDs on MRSA for 340 J at 30 min and 680 J at 60 min inhibited the growth of cell colonies. These findings support the use of photobiomodulation in Central venous port to prevent CV access port-Blood stream infection.

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来源期刊
Lasers in Medical Science
Lasers in Medical Science 医学-工程:生物医学
CiteScore
4.50
自引率
4.80%
发文量
192
审稿时长
3-8 weeks
期刊介绍: Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.
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