通过手术诱导髋臼过度覆盖,建立新型股骨髋臼撞击兔模型。

IF 2.1 3区 医学 Q2 ORTHOPEDICS
Siqi Zhang, Guanying Gao, Xiang Zhou, Cancan Du, Yichuan Zhu, Tong-Chuan He, Yan Xu
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引用次数: 0

摘要

股骨髋臼撞击症(FAI)可诱发关节内病变并导致骨关节炎(OA)恶化,但目前缺乏有效的小动物模型。FAI 患者关节软骨的基因表达谱尚未在动物实验中得到描述。本研究的目的是描述一种新型兔FAI模型,该模型可有效诱导关节内病变和OA进展,并利用该模型描述撞击软骨的基因表达模式。30 只 6 个月大的新西兰白兔接受了单侧髋臼边缘内扣植入手术,通过手术形成过度覆盖。放射学评估证实,所有接受手术的兔子都在髋臼边缘安全放置了内扣,外侧中心-边缘角(ΔLCEA)的平均变化为 16.2 ± 6.6°。大体检查显示,手术髋关节的股骨头前上方区域存在继发性软骨损伤。改良Outerbridge评分和Mankin评分显示,软骨损伤会随着撞击持续时间的延长而加重。免疫染色和定量实时聚合酶链反应显示,撞击软骨中炎症、合成代谢和分解代谢基因的表达量升高。软骨组织的 RNA 测序分析显示了独特的转录组特征,并确定 C-KIT、CD86 和 CD68 为中心标记物。我们的研究证实,新型兔FAI模型会造成髋臼过度覆盖,并在撞击区产生关节软骨损伤。来自撞击区的软骨表现出一种高度代谢状态,这与在 FAI 患者身上观察到的基因表达模式相吻合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a novel rabbit model for femoroacetabular impingement through surgically induced acetabular overcoverage.

There is a lack of validated small animal models for femoroacetabular impingement (FAI) that induce intra-articular lesions and cause osteoarthritis (OA) progression. The gene expression profile of articular cartilage in patients with FAI has not been characterized in animal studies. The purpose of this study is to describe a novel rabbit model for FAI with validated induction of intra-articular lesions and OA progression and to characterize the gene expression pattern in impinged cartilage using this model. Thirty 6-month-old New Zealand White rabbits underwent unilateral endobutton implant placement at the acetabular rim to surgically create overcoverage. Radiological assessment confirmed secure placement of endobutton at the acetabular rim for all operated hips with a mean alteration in lateral center-edge angle (ΔLCEA) of 16.2 ± 6.6°. Gross inspection revealed secondary cartilage injuries in the anterosuperior region of the femoral head for the operated hips. Cartilage injuries were shown to exacerbate with increased impingement duration, as demonstrated by the modified Outerbridge scores and Mankin scores. Immunostaining and quantitative real-time polymerase chain reaction revealed elevated expression of inflammatory, anabolic and catabolic genes in impinged cartilage. RNA sequencing analysis of cartilage tissue revealed a distinct transcriptome profile and identified C-KIT, CD86, and CD68 as central markers. Our study confirmed that the novel rabbit FAI model created acetabular overcoverage and produced articular cartilage injury at the impingement zone. Cartilage from the impingement zone demonstrated a heightened metabolic state, corroborating with the gene expression pattern observed in patients with FAI.

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来源期刊
Journal of Orthopaedic Research®
Journal of Orthopaedic Research® 医学-整形外科
CiteScore
6.10
自引率
3.60%
发文量
261
审稿时长
3-6 weeks
期刊介绍: The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.
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