Thomas VanderYacht, Liseth Salinas, Tilden Remerleitch, Nikolina Walas, Tin Ho, Gabriel Trueba, Jay P. Graham
{"title":"一个小镇缺乏废水处理,导致灌溉水中产生 ESBL 的大肠杆菌扩散。","authors":"Thomas VanderYacht, Liseth Salinas, Tilden Remerleitch, Nikolina Walas, Tin Ho, Gabriel Trueba, Jay P. Graham","doi":"10.1002/jeq2.20641","DOIUrl":null,"url":null,"abstract":"<p>Antibiotic resistance (ABR) is a critical and growing global challenge, especially in low- and middle-income countries. Ecuador has made great progress in connecting households to piped water supplies; however, the collection and treatment of domestic wastewater has lagged. This infrastructural gap may be accelerating the spread of ABR into surface waters used downstream for irrigation. We studied the contributions of a small town in Ecuador to the prevalence of extended-spectrum β-lactamase-producing <i>E</i>scherichia <i>coli</i> in a glacial stream used for irrigating crops. The study analyzed water samples upstream (<i>n</i> = 60) and downstream (<i>n</i> = 60) of the town of Píntag as well as 30 lettuce samples irrigated by surface waters downstream of the town. A subset of third generation cephalosporin resistant <i>E. coli</i> (3GCR-EC) isolates (<i>n</i> = 58) were sequenced to characterize antibiotic resistance genes and pathogenic lineages. Our results showed that there was nearly a three-log increase in mean <i>E. coli</i> colony forming units in the downstream samples versus upstream. At the upstream sites above the town of Píntag, 6.7% of water samples were positive for 3GCR-EC compared to 100% of samples collected at the downstream sites. Additionally, 70.1% of sequenced 3GCR-EC isolates collected at downstream sites carried <i>bla</i><sub>CTX-M</sub> genes and 3.4% belonged to pandemic lineages ST131 and ST10. As countries develop household piped water infrastructure, attention should focus on how the lack of domestic wastewater collection and treatment may accelerate the spread of ABR in waterways and the food system.</p>","PeriodicalId":15732,"journal":{"name":"Journal of environmental quality","volume":"53 6","pages":"1116-1126"},"PeriodicalIF":2.2000,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jeq2.20641","citationCount":"0","resultStr":"{\"title\":\"Lack of wastewater treatment in a small town drives the spread of ESBL-producing Escherichia coli in irrigation waters\",\"authors\":\"Thomas VanderYacht, Liseth Salinas, Tilden Remerleitch, Nikolina Walas, Tin Ho, Gabriel Trueba, Jay P. Graham\",\"doi\":\"10.1002/jeq2.20641\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Antibiotic resistance (ABR) is a critical and growing global challenge, especially in low- and middle-income countries. Ecuador has made great progress in connecting households to piped water supplies; however, the collection and treatment of domestic wastewater has lagged. This infrastructural gap may be accelerating the spread of ABR into surface waters used downstream for irrigation. We studied the contributions of a small town in Ecuador to the prevalence of extended-spectrum β-lactamase-producing <i>E</i>scherichia <i>coli</i> in a glacial stream used for irrigating crops. The study analyzed water samples upstream (<i>n</i> = 60) and downstream (<i>n</i> = 60) of the town of Píntag as well as 30 lettuce samples irrigated by surface waters downstream of the town. A subset of third generation cephalosporin resistant <i>E. coli</i> (3GCR-EC) isolates (<i>n</i> = 58) were sequenced to characterize antibiotic resistance genes and pathogenic lineages. Our results showed that there was nearly a three-log increase in mean <i>E. coli</i> colony forming units in the downstream samples versus upstream. At the upstream sites above the town of Píntag, 6.7% of water samples were positive for 3GCR-EC compared to 100% of samples collected at the downstream sites. Additionally, 70.1% of sequenced 3GCR-EC isolates collected at downstream sites carried <i>bla</i><sub>CTX-M</sub> genes and 3.4% belonged to pandemic lineages ST131 and ST10. As countries develop household piped water infrastructure, attention should focus on how the lack of domestic wastewater collection and treatment may accelerate the spread of ABR in waterways and the food system.</p>\",\"PeriodicalId\":15732,\"journal\":{\"name\":\"Journal of environmental quality\",\"volume\":\"53 6\",\"pages\":\"1116-1126\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jeq2.20641\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of environmental quality\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jeq2.20641\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of environmental quality","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jeq2.20641","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Lack of wastewater treatment in a small town drives the spread of ESBL-producing Escherichia coli in irrigation waters
Antibiotic resistance (ABR) is a critical and growing global challenge, especially in low- and middle-income countries. Ecuador has made great progress in connecting households to piped water supplies; however, the collection and treatment of domestic wastewater has lagged. This infrastructural gap may be accelerating the spread of ABR into surface waters used downstream for irrigation. We studied the contributions of a small town in Ecuador to the prevalence of extended-spectrum β-lactamase-producing Escherichia coli in a glacial stream used for irrigating crops. The study analyzed water samples upstream (n = 60) and downstream (n = 60) of the town of Píntag as well as 30 lettuce samples irrigated by surface waters downstream of the town. A subset of third generation cephalosporin resistant E. coli (3GCR-EC) isolates (n = 58) were sequenced to characterize antibiotic resistance genes and pathogenic lineages. Our results showed that there was nearly a three-log increase in mean E. coli colony forming units in the downstream samples versus upstream. At the upstream sites above the town of Píntag, 6.7% of water samples were positive for 3GCR-EC compared to 100% of samples collected at the downstream sites. Additionally, 70.1% of sequenced 3GCR-EC isolates collected at downstream sites carried blaCTX-M genes and 3.4% belonged to pandemic lineages ST131 and ST10. As countries develop household piped water infrastructure, attention should focus on how the lack of domestic wastewater collection and treatment may accelerate the spread of ABR in waterways and the food system.
期刊介绍:
Articles in JEQ cover various aspects of anthropogenic impacts on the environment, including agricultural, terrestrial, atmospheric, and aquatic systems, with emphasis on the understanding of underlying processes. To be acceptable for consideration in JEQ, a manuscript must make a significant contribution to the advancement of knowledge or toward a better understanding of existing concepts. The study should define principles of broad applicability, be related to problems over a sizable geographic area, or be of potential interest to a representative number of scientists. Emphasis is given to the understanding of underlying processes rather than to monitoring.
Contributions are accepted from all disciplines for consideration by the editorial board. Manuscripts may be volunteered, invited, or coordinated as a special section or symposium.