Calamintha nepeta L. 水提取物的体外生物活性。

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2024-09-01 Epub Date: 2024-09-05 DOI:10.32725/jab.2024.017
Nadia Salhi, Dorien Deluyker, Virginie Bito, Abdelhamid Zaid, Lhoussaine El Rhaffari
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引用次数: 0

摘要

目的:本研究旨在探讨石菖蒲叶水提取物的酚类成分、抗氧化能力、毒性及其潜在的血管舒张作用:方法:采用煎煮法、浸泡法和浸渍法三种提取方法制备石菖蒲水提取物。研究了提取物的总酚含量及其抗氧化性。毒性采用盐水蒿致死生物测定法进行评估。煎煮提取物的化学成分通过 HPLC 进行分析,并用于评估其对从健康 Sprague Dawley 大鼠身上分离的胸主动脉环的血管舒张作用。先用苯肾上腺素诱导预收缩,再用累积剂量的提取物(0.001 至 250 µg/ml)诱导预收缩:Calamintha nepeta L.的水提取物具有明显的自由基清除和螯合活性。不过,煎煮提取物的抗氧化能力最强。煎煮和浸泡提取物均无毒性。咖啡酸、槲皮素和迷迭香酸是主要的鉴定化合物。值得注意的是,煎煮法提取的水提取物在较低浓度下可诱导内皮细胞接触的主动脉环明显松弛,在较高浓度下可诱导变性主动脉环明显松弛:本研究揭示了用煎煮法提取的肾形石菖蒲具有强大的抗氧化能力,并具有依赖于内皮的血管舒张作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro biological activities of Calamintha nepeta L. aqueous extracts.

Aim: This study aimed to investigate the phenolic composition, antioxidant capacity, and toxicity of aqueous extracts of Calamintha nepeta L. leaves and their potential vasorelaxant effects.

Methods: Aqueous extracts of Calamintha nepeta L. were prepared by three extraction methods: decoction, infusion, and maceration. The total phenolic contents of the extracts and their antioxidant properties were investigated. The toxicity was evaluated by Artemia salina lethality bioassay. The decoction extract was analyzed by HPLC for its chemical profile and was also used to evaluate the vasorelaxant effect on thoracic aortic rings isolated from healthy Sprague Dawley rats. Pre-contraction was induced by phenylephrine, followed by cumulative doses of the extract (0.001 up to 250 µg/ml).

Results: Aqueous extracts of Calamintha nepeta L. showed noticeable radical scavenging and chelating activities. However, the decoction extract exhibited the most powerful antioxidant capacity. No toxicity was recorded for the extracts obtained by decoction and infusion. Caffeic acid, quercetin, and rosmarinic acid were the main identified compounds. Notably, the aqueous extract obtained by decoction induced significant relaxation in endothelium-intact aortic rings at lower concentrations, and at higher concentrations in denuded aortic rings.

Conclusion: This study reveals that Calamintha nepeta L. extracted with a decoction method possesses potent antioxidant capacity and has an endothelium-dependent vasorelaxant effect.

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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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