与大肠杆菌共存的细菌氨基酰-tRNA 合成酶同系物表现出不同的系统发育背景和功能兼容性。

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
IUBMB Life Pub Date : 2024-10-17 DOI:10.1002/iub.2920
Alexander A. Radecki, Ariana Fantasia-Davis, Juan S. Maldonado, Joshua W. Mann, Stephanie Sepulveda-Camacho, Pearl Morosky, Jordan Douglas, Oscar Vargas-Rodriguez
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引用次数: 0

摘要

氨基酰-tRNA 合成酶(aaRS)是合成蛋白质合成所需的氨基酰-tRNA 底物的普遍必需酶。虽然大多数生物体的每个蛋白质氨基酸都需要一个 aaRS 基因来翻译其遗传信息,但许多物种都编码多个 aaRS 基因拷贝。越来越多的证据表明,生物会获得额外的 aaRS 基因,以维持或适应其独特的生活方式。然而,由于重复 aaRS 基因在宿主生物体中可能具有独特的生理作用,而且文献中对重复 aaRS 基因的注释并不一致,因此预测和定义重复 aaRS 基因的功能仍然具有挑战性。在此,我们进行了比较、系统发育和功能研究,以确定在几种人类致病菌中编码的色氨酰、酪氨酸、丝氨酸和脯氨酰-tRNA 合成酶共存旁系亲和体的活性。我们的分析表明,除少数例外情况外,重复的 aaRS 涉及具有不同系统发育背景的旁系基因。通过收集大肠杆菌菌株,我们发现几乎在所有情况下,一种 aaRS 具有转运核糖核酸(tRNA)氨基酰化活性,而另一种则与大肠杆菌不相容。总之,这项工作说明了鉴定、分类和预测 aaRS 旁系亲属功能所面临的挑战,并凸显了 aaRS 进化的复杂性。此外,这些结果为了解 aaRS 旁系亲属在几种人类病原体生物学中的潜在作用提供了新的视角,也为研究细菌中重复的 aaRS 旁系亲属的生理作用提供了基础知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Coexisting bacterial aminoacyl-tRNA synthetase paralogs exhibit distinct phylogenetic backgrounds and functional compatibility with Escherichia coli

Aminoacyl-tRNA synthetases (aaRSs) are universally essential enzymes that synthesize aminoacyl-tRNA substrates for protein synthesis. Although most organisms require a single aaRS gene for each proteinogenic amino acid to translate their genetic information, numerous species encode multiple gene copies of an aaRS. Growing evidence indicates that organisms acquire extra aaRS genes to sustain or adapt to their unique lifestyle. However, predicting and defining the function of repeated aaRS genes remains challenging due to their potentially unique physiological role in the host organism and the inconsistent annotation of repeated aaRS genes in the literature. Here, we carried out comparative, phylogenetic, and functional studies to determine the activity of coexisting paralogs of tryptophanyl-, tyrosyl-, seryl-, and prolyl-tRNA synthetases encoded in several human pathogenic bacteria. Our analyses revealed that, with a few exceptions, repeated aaRSs involve paralogous genes with distinct phylogenetic backgrounds. Using a collection of Escherichia coli strains that enabled the facile characterization of aaRS activity in vivo, we found that, in almost all cases, one aaRS displayed transfer RNA (tRNA) aminoacylation activity, whereas the other was not compatible with E. coli. Together, this work illustrates the challenges of identifying, classifying, and predicting the function of aaRS paralogs and highlights the complexity of aaRS evolution. Moreover, these results provide new insights into the potential role of aaRS paralogs in the biology of several human pathogens and foundational knowledge for the investigation of the physiological role of repeated aaRS paralogs across bacteria.

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来源期刊
IUBMB Life
IUBMB Life 生物-生化与分子生物学
CiteScore
10.60
自引率
0.00%
发文量
109
审稿时长
4-8 weeks
期刊介绍: IUBMB Life is the flagship journal of the International Union of Biochemistry and Molecular Biology and is devoted to the rapid publication of the most novel and significant original research articles, reviews, and hypotheses in the broadly defined fields of biochemistry, molecular biology, cell biology, and molecular medicine.
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