{"title":"从北冰洋表层沉积物中分离的 Thioclava arctica sp.","authors":"Panyi Pan, Jianning Wang, Xiupian Liu, Qiliang Lai, Guangyu Li, Yaping Du, Jinzhu Zhang, Ziyuan He, Zongze Shao, Chunming Dong","doi":"10.1099/ijsem.0.006548","DOIUrl":null,"url":null,"abstract":"<p><p>Two Gram-stain-negative bacterial strains, 15-R06ZXC-3<sup>T</sup> and R06B22<sup>T</sup>, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3<sup>T</sup> belongs to the genus <i>Thioclava</i>, while strain R06B22<sup>T</sup> belongs to the genus <i>Zhongshania</i>. Strain 15-R06ZXC-3<sup>T</sup> showed the closest relationship to <i>Thioclava indica</i> DT23-4<sup>T</sup>. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3<sup>T</sup> and all of the type strains of the genus <i>Thioclava</i> ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22<sup>T</sup> was most closely related to <i>Zhongshania marina</i> DSW25-10<sup>T</sup>. The dDDH and ANI values between strain R06B22<sup>T</sup> and all of the type strains of the genus <i>Zhongshania</i> ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3<sup>T</sup> and R06B22<sup>T</sup> separately represent novel species of the genera <i>Thioclava</i> and <i>Zhongshania</i>, for which the names <i>Thioclava arctica</i> sp. nov. (type strain 15-R06ZXC-3<sup>T</sup> = MCCC 1A07434<sup>T</sup>= KCTC 8342<sup>T</sup>) and <i>Zhongshania arctica</i> sp. nov. (type strain R06B22<sup>T</sup> = MCCC 1A08273<sup>T</sup>= KCTC 8343<sup>T</sup>) are proposed. Additionally, phylogenomic analyses showed that the strain <i>Marortus luteolus</i> ZX-21<sup>T</sup> was clustered with the strain <i>Z. marina</i> DSW25-10<sup>T</sup> and all other type strains of the genus <i>Zhongshania</i>. Furthermore, the ANI and dDDH values between strains ZX-21<sup>T</sup> and DSW25-10<sup>T</sup> were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that <i>M. luteolus</i> Yu <i>et al.</i> 2019 be recognized as a later heterotypic synonym of <i>Z. marina</i> On <i>et al.</i> 2019.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Thioclava arctica</i> sp. nov. and <i>Zhongshania arctica</i> sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of <i>Marortus luteolus</i> Yu <i>et al.</i> 2019 as a later heterotypic synonym of <i>Zhongshania marina</i> On <i>et al.</i> 2019.\",\"authors\":\"Panyi Pan, Jianning Wang, Xiupian Liu, Qiliang Lai, Guangyu Li, Yaping Du, Jinzhu Zhang, Ziyuan He, Zongze Shao, Chunming Dong\",\"doi\":\"10.1099/ijsem.0.006548\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Two Gram-stain-negative bacterial strains, 15-R06ZXC-3<sup>T</sup> and R06B22<sup>T</sup>, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3<sup>T</sup> belongs to the genus <i>Thioclava</i>, while strain R06B22<sup>T</sup> belongs to the genus <i>Zhongshania</i>. Strain 15-R06ZXC-3<sup>T</sup> showed the closest relationship to <i>Thioclava indica</i> DT23-4<sup>T</sup>. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3<sup>T</sup> and all of the type strains of the genus <i>Thioclava</i> ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22<sup>T</sup> was most closely related to <i>Zhongshania marina</i> DSW25-10<sup>T</sup>. The dDDH and ANI values between strain R06B22<sup>T</sup> and all of the type strains of the genus <i>Zhongshania</i> ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3<sup>T</sup> and R06B22<sup>T</sup> separately represent novel species of the genera <i>Thioclava</i> and <i>Zhongshania</i>, for which the names <i>Thioclava arctica</i> sp. nov. (type strain 15-R06ZXC-3<sup>T</sup> = MCCC 1A07434<sup>T</sup>= KCTC 8342<sup>T</sup>) and <i>Zhongshania arctica</i> sp. nov. (type strain R06B22<sup>T</sup> = MCCC 1A08273<sup>T</sup>= KCTC 8343<sup>T</sup>) are proposed. Additionally, phylogenomic analyses showed that the strain <i>Marortus luteolus</i> ZX-21<sup>T</sup> was clustered with the strain <i>Z. marina</i> DSW25-10<sup>T</sup> and all other type strains of the genus <i>Zhongshania</i>. Furthermore, the ANI and dDDH values between strains ZX-21<sup>T</sup> and DSW25-10<sup>T</sup> were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that <i>M. luteolus</i> Yu <i>et al.</i> 2019 be recognized as a later heterotypic synonym of <i>Z. marina</i> On <i>et al.</i> 2019.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006548\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006548","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Thioclava arctica sp. nov. and Zhongshania arctica sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of Marortus luteolus Yu et al. 2019 as a later heterotypic synonym of Zhongshania marina On et al. 2019.
Two Gram-stain-negative bacterial strains, 15-R06ZXC-3T and R06B22T, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3T belongs to the genus Thioclava, while strain R06B22T belongs to the genus Zhongshania. Strain 15-R06ZXC-3T showed the closest relationship to Thioclava indica DT23-4T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3T and all of the type strains of the genus Thioclava ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22T was most closely related to Zhongshania marina DSW25-10T. The dDDH and ANI values between strain R06B22T and all of the type strains of the genus Zhongshania ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3T and R06B22T separately represent novel species of the genera Thioclava and Zhongshania, for which the names Thioclava arctica sp. nov. (type strain 15-R06ZXC-3T = MCCC 1A07434T= KCTC 8342T) and Zhongshania arctica sp. nov. (type strain R06B22T = MCCC 1A08273T= KCTC 8343T) are proposed. Additionally, phylogenomic analyses showed that the strain Marortus luteolus ZX-21T was clustered with the strain Z. marina DSW25-10T and all other type strains of the genus Zhongshania. Furthermore, the ANI and dDDH values between strains ZX-21T and DSW25-10T were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that M. luteolus Yu et al. 2019 be recognized as a later heterotypic synonym of Z. marina On et al. 2019.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
