血管内皮生长因子/结缔组织生长因子和蛋白质组学分析用于增殖性糖尿病视网膜病变的玻璃体内康柏西普治疗后的房水。

IF 1.9 4区 医学 Q2 OPHTHALMOLOGY
International journal of ophthalmology Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI:10.18240/ijo.2024.10.07
Hou-Shuo Li, Xiao Lyu, Ao Rong, Yan-Long Bi, Wei Xu, Hong-Ping Cui
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引用次数: 0

摘要

目的:探讨增殖性糖尿病视网膜病变(PDR)患者在玻璃体内注射康柏西普之后,结缔组织生长因子(CTGF)和血管内皮生长因子(VEGF)在房水蛋白质谱中的作用:这项研究包括72名增殖性糖尿病视网膜病变患者和8名白内障患者作为对照组。根据玻璃体内注射康柏西普(IVC,0.5 毫克/0.05 毫升)与进行玻璃体旁切除术(PPV)之间的 3、5 和 7 天间隔,将 PDR 患者分为 3 组。在玻璃体内注射康柏西汀(IVC,0.5 毫克/0.05 毫升)和玻璃体旁切除术(PPV)前后采集了眼房水样本,用酶联免疫吸附试验(ELISA)检测血管内皮生长因子和 CTGF 水平。研究了IVC术后5d接受PPV手术的10名患者和8名正常对照组的差异蛋白质组学,对数据进行了基因本体(GO)和京都基因组百科全书(KEGG)分析,并研究了23种差异蛋白质的蛋白质相互作用网络:结果:IVC后,眼房水中VEGF水平显著下降,CTGF水平显著上升,CTGF/VEGF比值在所有时间间隔内均显著上升。液相色谱串联质谱法(LC-MS/MS)确定了IVC前后样本中表达不同的蛋白质。GO 和 KEGG 分析显示,这些蛋白参与了免疫反应、应激反应、补体和凝血级联、铁蛋白沉积和 PPAR 信号通路。PPI分析强调了APOA1、C3和转铁蛋白(TF)等关键蛋白。ELISA 检测证实了 HBA1、SERPINA1、COL1A1 和 ACTB 等蛋白质的不同表达,在 IVC 组中有显著变化:研究表明,IVC 能有效降低血管内皮生长因子的水平,同时提高 CTGF 的水平,从而改变 CTGF/VEGF 的比例。蛋白质组分析表明,这些变化与涉及免疫反应、应激反应和细胞代谢的关键生物通路和蛋白质相互作用有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vascular endothelial growth factor/connective tissue growth factor and proteomic analysis of aqueous humor after intravitreal conbercept for proliferative diabetes retinopathy.

Aim: To investigate the role of connective tissue growth factor (CTGF) and vascular endothelial growth factor (VEGF) in the protein profile of the aqueous humor in patients with proliferative diabetic retinopathy (PDR) following intravitreal injection of conbercept.

Methods: This study included 72 PDR patients and 8 cataract patients as controls. PDR patients were divided into 3 groups according to the intervals of 3, 5, and 7d between intravitreal conbercept (IVC, 0.5 mg/0.05 mL) injection and pars plana vitrectomy (PPV) performed. Aqueous humor samples were collected before and after IVC and PPV for VEGF and CTGF levels detected with enzyme-linked immunosorbent assay (ELISA). The differential proteomics of 10 patients who underwent PPV surgery 5d after IVC and 8 normal controls was studied, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on the data, and the protein interaction network of 23 differential proteins was studied.

Results: Post-IVC, VEGF levels decreased and CTGF levels increased significantly in aqueous humor, with the CTGF/VEGF ratio rising significantly at all intervals. Liquid chromatography tandem mass spectrometry (LC-MS/MS) identified differentially expressed proteins between pre- and post-IVC samples. GO and KEGG analyses revealed involvement in immune response, stress response, complement and coagulation cascades, ferroptosis, and PPAR signaling pathways. PPI analysis highlighted key proteins like APOA1, C3, and transferrin (TF). ELISA assay confirmed the differential expression of proteins such as HBA1, SERPINA1, COL1A1, and ACTB, with significant changes in the IVC groups.

Conclusion: The study demonstrates that IVC effectively reduces VEGF levels while increasing CTGF levels, thereby modifying the CTGF/VEGF ratio, and IVC significantly alters the protein profile in the aqueous humor of patients with PDR. Proteomic analysis reveals that these changes are associated with critical biological pathways and protein interactions involved in immune response, stress response, and cellular metabolism.

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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
3141
审稿时长
4-8 weeks
期刊介绍: · International Journal of Ophthalmology-IJO (English edition) is a global ophthalmological scientific publication and a peer-reviewed open access periodical (ISSN 2222-3959 print, ISSN 2227-4898 online). This journal is sponsored by Chinese Medical Association Xi’an Branch and obtains guidance and support from WHO and ICO (International Council of Ophthalmology). It has been indexed in SCIE, PubMed, PubMed-Central, Chemical Abstracts, Scopus, EMBASE , and DOAJ. IJO JCR IF in 2017 is 1.166. IJO was established in 2008, with editorial office in Xi’an, China. It is a monthly publication. General Scientific Advisors include Prof. Hugh Taylor (President of ICO); Prof.Bruce Spivey (Immediate Past President of ICO); Prof.Mark Tso (Ex-Vice President of ICO) and Prof.Daiming Fan (Academician and Vice President, Chinese Academy of Engineering. International Scientific Advisors include Prof. Serge Resnikoff (WHO Senior Speciatist for Prevention of blindness), Prof. Chi-Chao Chan (National Eye Institute, USA) and Prof. Richard L Abbott (Ex-President of AAO/PAAO) et al. Honorary Editors-in-Chief: Prof. Li-Xin Xie(Academician of Chinese Academy of Engineering/Honorary President of Chinese Ophthalmological Society); Prof. Dennis Lam (President of APAO) and Prof. Xiao-Xin Li (Ex-President of Chinese Ophthalmological Society). Chief Editor: Prof. Xiu-Wen Hu (President of IJO Press). Editors-in-Chief: Prof. Yan-Nian Hui (Ex-Director, Eye Institute of Chinese PLA) and Prof. George Chiou (Founding chief editor of Journal of Ocular Pharmacology & Therapeutics). Associate Editors-in-Chief include: Prof. Ning-Li Wang (President Elect of APAO); Prof. Ke Yao (President of Chinese Ophthalmological Society) ; Prof.William Smiddy (Bascom Palmer Eye instituteUSA) ; Prof.Joel Schuman (President of Association of University Professors of Ophthalmology,USA); Prof.Yizhi Liu (Vice President of Chinese Ophtlalmology Society); Prof.Yu-Sheng Wang (Director of Eye Institute of Chinese PLA); Prof.Ling-Yun Cheng (Director of Ocular Pharmacology, Shiley Eye Center, USA). IJO accepts contributions in English from all over the world. It includes mainly original articles and review articles, both basic and clinical papers. Instruction is Welcome Contribution is Welcome Citation is Welcome Cooperation organization International Council of Ophthalmology(ICO), PubMed, PMC, American Academy of Ophthalmology, Asia-Pacific, Thomson Reuters, The Charlesworth Group, Crossref,Scopus,Publons, DOAJ etc.
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