Joseph Arballo, Jennifer M Rutkowsky, Marjorie J Haskell, Kyla De Las Alas, Reina Engle-Stone, Xiaogu Du, Jon J Ramsey, Peng Ji
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引用次数: 0
摘要
在发展中国家,孕妇和儿童很容易缺乏维生素 A(VAD),而同时缺乏其他微量营养素(尤其是铁和锌)往往会加重这种情况。这项研究在小鼠模型中调查了生命早期维生素 A 缺乏对小鼠运动和认知发展以及微量元素状况的影响。C57BL/6J 母鼠在两个连续的妊娠期和哺乳期内分别摄入维生素 A 充足(VR)或缺乏(VD)的饮食。两个妊娠期的后代(G1 和 G2)在 6 周龄或 9 周龄前一直食用相同的食物。进行了行为测定,以评估运动协调性、握力、空间认知和焦虑。对肝脏微量元素进行了分析。在所有年龄和妊娠期,VD饮食都会消耗肝脏中的视黄醇,并降低血浆中的视黄醇含量。后腿后缩和步态异常是两个妊娠期的VD后代在9周时最常见的临床表现。在旋转木马测试中的不良表现进一步证实了他们的运动功能障碍。VAD 不影响血红蛋白水平,对肝脏微量矿物质浓度也没有一致的影响。这些发现凸显了维生素 A 在运动发育中的关键作用。目前还没有明确的证据表明,VAD会改变缺铁性贫血或微量元素的风险。
Pre- and Postnatal Vitamin A Deficiency Impairs Motor Skills without a Consistent Effect on Trace Mineral Status in Young Mice.
Pregnant women and children are vulnerable to vitamin A deficiency (VAD), which is often compounded by concurrent deficiencies in other micronutrients, particularly iron and zinc, in developing countries. The study investigated the effects of early-life VAD on motor and cognitive development and trace mineral status in a mouse model. C57BL/6J dams were fed either a vitamin A-adequate (VR) or -deficient (VD) diet across two consecutive gestations and lactations. Offspring from both gestations (G1 and G2) continued the same diets until 6 or 9 weeks of age. Behavioral assays were conducted to evaluate motor coordination, grip strength, spatial cognition, and anxiety. Hepatic trace minerals were analyzed. A VD diet depleted hepatic retinoids and reduced plasma retinol across all ages and gestations. Retracted rear legs and abnormal gait were the most common clinical manifestations observed in VD offspring from both gestations at 9 weeks. Poor performance on the Rotarod test further confirmed their motor dysfunction. VAD didn't affect hemoglobin levels and had no consistent effect on hepatic trace mineral concentrations. These findings highlight the critical role of vitamin A in motor development. There was no clear evidence that VAD alters the risk of iron deficiency anemia or trace minerals.
期刊介绍:
The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).