Zahra Mirabedini, Maryam Niyyati, Hanieh Mohammad Rahimi, Sara Soleimani Jevinani, Marziye Fatemi, Mohammad Tanhaei, Seyed Reza Mohebbi, Abbas Yadegar, Sara Abolghasemi, Zahra Arab Mazar, Hamed Mirjalali
{"title":"从 COVID-19 患者体内分离出的自由生活变形虫中存在酵母菌和细菌：担心继发感染。","authors":"Zahra Mirabedini, Maryam Niyyati, Hanieh Mohammad Rahimi, Sara Soleimani Jevinani, Marziye Fatemi, Mohammad Tanhaei, Seyed Reza Mohebbi, Abbas Yadegar, Sara Abolghasemi, Zahra Arab Mazar, Hamed Mirjalali","doi":"10.1080/09603123.2024.2409830","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs (<i>n</i> = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated. From 60 COVID-19 samples, 18 (30%) were positive for FLA. <i>Acanthamoeba</i> sp. <i>Naegleria australiensis</i>, <i>Tetramitus</i> sp. and <i>Vermamoeba vermiformis</i> were characterized in 12 (80%), 1 (6.66%), 2 (13.33%), and 7 (38.88%) of samples, respectively. SARS-CoV-2 RNA was not detected in FLA. <i>Candida albicans</i>, <i>C. tropicalis</i>, and <i>C. parapsilosis</i> were detected in (11/18; 61.11%), (3/18; 16.67%), and (3/18; 16.67%) of samples, respectively. <i>Geotrichum candidum</i> was detected in 10/18 (55.55%) of samples. <i>Streptococcus</i> spp. and <i>Staphylococcus</i> spp. were identified in 16/18 (88.88%) and 3/18 (16.67%), respectively. The presence of yeasts and bacteria signifies the possible role of FLA in distribution of secondary infections in susceptible patients.</p>","PeriodicalId":14039,"journal":{"name":"International Journal of Environmental Health Research","volume":" ","pages":"1-14"},"PeriodicalIF":2.2000,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The presence of yeasts and bacteria in free-living amoebae isolated from COVID-19 patients: concern for secondary infections.\",\"authors\":\"Zahra Mirabedini, Maryam Niyyati, Hanieh Mohammad Rahimi, Sara Soleimani Jevinani, Marziye Fatemi, Mohammad Tanhaei, Seyed Reza Mohebbi, Abbas Yadegar, Sara Abolghasemi, Zahra Arab Mazar, Hamed Mirjalali\",\"doi\":\"10.1080/09603123.2024.2409830\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs (<i>n</i> = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated. From 60 COVID-19 samples, 18 (30%) were positive for FLA. <i>Acanthamoeba</i> sp. <i>Naegleria australiensis</i>, <i>Tetramitus</i> sp. and <i>Vermamoeba vermiformis</i> were characterized in 12 (80%), 1 (6.66%), 2 (13.33%), and 7 (38.88%) of samples, respectively. SARS-CoV-2 RNA was not detected in FLA. <i>Candida albicans</i>, <i>C. tropicalis</i>, and <i>C. parapsilosis</i> were detected in (11/18; 61.11%), (3/18; 16.67%), and (3/18; 16.67%) of samples, respectively. <i>Geotrichum candidum</i> was detected in 10/18 (55.55%) of samples. <i>Streptococcus</i> spp. and <i>Staphylococcus</i> spp. were identified in 16/18 (88.88%) and 3/18 (16.67%), respectively. The presence of yeasts and bacteria signifies the possible role of FLA in distribution of secondary infections in susceptible patients.</p>\",\"PeriodicalId\":14039,\"journal\":{\"name\":\"International Journal of Environmental Health Research\",\"volume\":\" \",\"pages\":\"1-14\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Environmental Health Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/09603123.2024.2409830\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Health Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09603123.2024.2409830","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
The presence of yeasts and bacteria in free-living amoebae isolated from COVID-19 patients: concern for secondary infections.
This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs (n = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated. From 60 COVID-19 samples, 18 (30%) were positive for FLA. Acanthamoeba sp. Naegleria australiensis, Tetramitus sp. and Vermamoeba vermiformis were characterized in 12 (80%), 1 (6.66%), 2 (13.33%), and 7 (38.88%) of samples, respectively. SARS-CoV-2 RNA was not detected in FLA. Candida albicans, C. tropicalis, and C. parapsilosis were detected in (11/18; 61.11%), (3/18; 16.67%), and (3/18; 16.67%) of samples, respectively. Geotrichum candidum was detected in 10/18 (55.55%) of samples. Streptococcus spp. and Staphylococcus spp. were identified in 16/18 (88.88%) and 3/18 (16.67%), respectively. The presence of yeasts and bacteria signifies the possible role of FLA in distribution of secondary infections in susceptible patients.
期刊介绍：
International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.
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