评估早期和晚期子痫前期的人类胎盘微生物群。

IF 3.1 Q2 PERIPHERAL VASCULAR DISEASE
Kehinde S Olaniyi, Irene Mackraj, Jagidesa Moodley, Roshila Moodley
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引用次数: 0

摘要

导言:尽管经过几十年的研究,先兆子痫(PE)的确切病因仍然不明。人们提出了几种病因,包括胎盘微生物群的作用。目的:本研究旨在确定早期和晚期子痫妇女胎盘中共存的不同微生物:方法:分别招募了 30 名年龄匹配的正常血压和早发性以及年龄匹配的正常血压和晚发性子痫前期妇女。在获得知情同意后,通过无菌和标准化临床程序进行剖腹产获取胎盘组织。从每个组织中提取 DNA,采用 16 S 目标分析法进行微生物组分析,并对读数进行生物信息学分析:结果:与年龄匹配的正常血压对照组相比,早发/迟发 PE 患者的血压差异显著。此外,胎盘样本的读数被归类为放线菌门、固执菌门、类杆菌门、蛋白菌门,其中蛋白菌门以假单胞菌纲和伽马蛋白菌纲为主,放线菌门和类杆菌门的数量较少。与年龄匹配的正常血压对照组相比,早发型/晚发型 PE 胎盘细菌种类无明显差异。进一步分析发现,细菌种类与早发或晚发 PE 之间没有相关性:本研究结果表明,胎盘样本中的细菌种类生物量较低,这可能进一步表明胎盘样本中的细菌种类水平很低,而且细菌组成与早发或晚发 PE 之间没有相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the Human Placental Microbiota in Early- and Late-Onset Pre-Eclampsia.

Introduction: Despite many decades of research, the exact etiology of pre-eclampsia (PE) remains unknown. Several etiopathologies have been suggested, including the role of the placental microbiota. However, the existence of placental microbiota and its possible contribution to pregnancy complications, particularly PE has remained controversial.

Aim: The present study was designed to identify different microbes that co-exist the placenta of women with early- and late-onset PE.

Methods: Thirty age-matched normotensive and early-onset as well as age-matched normotensive and late-onset pre-eclamptic women respectively, were recruited. After obtaining an informed consent, the placental tissues were obtained through caesarian section with sterile and standardized clinical procedures. DNA was extracted from each tissue and microbiome analysis was conducted using a targeted 16 S analysis and the reads were analyzed with bioinformatics.

Results: There was a significance difference between the blood pressure of early-/late-onset PE compared with age-matched normotensive controls, respectively. In addition, the reads from placencental samples were classified as belonging to the phyla, Actinobacteria, Firmicutes, Bacteroidetes, Proteobacteria, with Proteobacteria dominated by the classes Pseudomonadales and Gammaproteobacteria with smaller amounts of Actinobacteria and Bacteroidetes. There was no significant difference between the placental bacterial species of early-/late-onset PE compared with age-matched normotensive controls, respectively. Further analysis found no correlation between bacterial species and early- or late-onset PE.

Conclusion: The present results demonstrate a low biomass of bacterial species, which might further indicate that the placental samples had very low levels of bacteria species and there is no correlation between the bacterial composition and early- or late-onset PE.

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来源期刊
CiteScore
5.70
自引率
3.30%
发文量
57
期刊介绍: High Blood Pressure & Cardiovascular Prevention promotes knowledge, update and discussion in the field of hypertension and cardiovascular disease prevention, by providing a regular programme of independent review articles covering key aspects of the management of hypertension and cardiovascular diseases. The journal includes:   Invited ''State of the Art'' reviews.  Expert commentaries on guidelines, major trials, technical advances.Presentation of new intervention trials design.''Pros and Cons'' or round tables on controversial issues.Statements on guidelines from hypertension and cardiovascular scientific societies.Socio-economic issues.Cost/benefit in prevention of cardiovascular diseases.Monitoring of healthcare systems.News and views from the Italian Society of Hypertension (including abstracts).All manuscripts are subject to peer review by international experts. Letters to the editor are welcomed and will be considered for publication.
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