Foxp3+ 调节性 T 细胞驻留在角膜上皮内，并与角膜缘干细胞共定位。

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2024-10-11 DOI:10.1016/j.exer.2024.110123

Maryam Tahvildari , Rao Me , Mizumi Setia , Nan Gao , Pratima Suvas , Sharon A. McClellan , Susmit Suvas

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引用次数: 0

摘要

在这项研究中，我们调查了角膜内常驻 Foxp3+ 调节性 T 细胞（Tregs）的存在情况，并评估了常驻 Tregs 在角膜上皮伤口愈合中的作用。通过小鼠模型，我们发现在稳定状态下，Foxp3+Tregs与ABCG2+角膜缘干细胞接近或共定位。我们还发现，这些 Tregs 驻留在上皮层而非角膜基质中。此外，我们利用小鼠机械损伤模型证明，在角膜机械损伤之前，通过结膜下注射抗CD25，从角膜中清除Tregs与角膜上皮愈合延迟有关。这些结果表明角膜常住Tregs在角膜上皮细胞功能和伤口愈合中的作用，为进一步探索Tregs在角膜缘干细胞功能和存活中的作用打开了大门。

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Foxp3+ regulatory T cells reside within the corneal epithelium and co-localize with limbal stem cells

In this study we investigated the presence of resident Foxp3⁺ regulatory T cells (Tregs) within the cornea and assessed the role of resident Tregs in corneal epithelial wound healing. Using a mouse model, we showed that in the steady state Foxp3⁺Tregs are either in close proximity or co-localize with ABCG2⁺ limbal stem cells. We also showed that these Tregs reside within the epithelial layer and not the corneal stroma. In addition, using a mouse model of mechanical injury, we demonstrated that depletion of Tregs from the cornea prior to corneal mechanical injury, using subconjunctival injection of anti-CD25, was associated with delayed epithelial healing. These results suggest a role for cornea resident Tregs in corneal epithelial cell function and wound healing and opens doors for further exploration of the role of Tregs in limbal stem cell function and survival.

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.