Vanessa Spieß, Rannyele P. Ribeiro, Conrad Helm, M. Teresa Aguado
{"title":"从两节到更长:调查马拉喹鲤的再生起始阶段。","authors":"Vanessa Spieß, Rannyele P. Ribeiro, Conrad Helm, M. Teresa Aguado","doi":"10.1111/ede.12492","DOIUrl":null,"url":null,"abstract":"<p>Annelids feature a diverse range of regenerative abilities, but complete whole-body regeneration is less common, particularly in the context of the head and anterior body regeneration. This study provides a detailed morphological description of <i>Syllis malaquini</i> regenerative abilities. By replicating previous experiments and performing diverse surgical procedures, we explored the capacity of this species for whole-body regeneration. We detailed the precise timing of regeneration of particular structures such as the eyes, proventricle, pharyngeal tooth, nuchal organs, and body pigmentation after amputation. Our high-resolution scanning electron microscopy and confocal laser-scanning microscopy images provide details of the blastema region, revealing that while anal opening remains in connection to the exterior environment, oral opening is formed “<i>de novo</i>” during blastema differentiation. Additionally, we performed amputations to isolate fragments consisting of one, two, and three segments from the intestinal trunk region. We found that <i>S. malaquini</i> requires at least two to three segments to successfully regenerate the whole body. In addition, we verified a variable capacity to regenerate depending upon the gut region, with structures of the foregut greatly impairing some steps of the regenerative process. Our work notably addresses the gap in knowledge concerning gut formation and its impact on regenerative capabilities. Ongoing research is crucial to unravel the role of gut tissue specificity and plasticity during regeneration in annelids, and particularly in syllids.</p>","PeriodicalId":12083,"journal":{"name":"Evolution & Development","volume":"26 6","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ede.12492","citationCount":"0","resultStr":"{\"title\":\"From two segments and beyond: Investigating the onset of regeneration in Syllis malaquini\",\"authors\":\"Vanessa Spieß, Rannyele P. Ribeiro, Conrad Helm, M. Teresa Aguado\",\"doi\":\"10.1111/ede.12492\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Annelids feature a diverse range of regenerative abilities, but complete whole-body regeneration is less common, particularly in the context of the head and anterior body regeneration. This study provides a detailed morphological description of <i>Syllis malaquini</i> regenerative abilities. By replicating previous experiments and performing diverse surgical procedures, we explored the capacity of this species for whole-body regeneration. We detailed the precise timing of regeneration of particular structures such as the eyes, proventricle, pharyngeal tooth, nuchal organs, and body pigmentation after amputation. Our high-resolution scanning electron microscopy and confocal laser-scanning microscopy images provide details of the blastema region, revealing that while anal opening remains in connection to the exterior environment, oral opening is formed “<i>de novo</i>” during blastema differentiation. Additionally, we performed amputations to isolate fragments consisting of one, two, and three segments from the intestinal trunk region. We found that <i>S. malaquini</i> requires at least two to three segments to successfully regenerate the whole body. In addition, we verified a variable capacity to regenerate depending upon the gut region, with structures of the foregut greatly impairing some steps of the regenerative process. Our work notably addresses the gap in knowledge concerning gut formation and its impact on regenerative capabilities. Ongoing research is crucial to unravel the role of gut tissue specificity and plasticity during regeneration in annelids, and particularly in syllids.</p>\",\"PeriodicalId\":12083,\"journal\":{\"name\":\"Evolution & Development\",\"volume\":\"26 6\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ede.12492\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Evolution & Development\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ede.12492\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Evolution & Development","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ede.12492","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
From two segments and beyond: Investigating the onset of regeneration in Syllis malaquini
Annelids feature a diverse range of regenerative abilities, but complete whole-body regeneration is less common, particularly in the context of the head and anterior body regeneration. This study provides a detailed morphological description of Syllis malaquini regenerative abilities. By replicating previous experiments and performing diverse surgical procedures, we explored the capacity of this species for whole-body regeneration. We detailed the precise timing of regeneration of particular structures such as the eyes, proventricle, pharyngeal tooth, nuchal organs, and body pigmentation after amputation. Our high-resolution scanning electron microscopy and confocal laser-scanning microscopy images provide details of the blastema region, revealing that while anal opening remains in connection to the exterior environment, oral opening is formed “de novo” during blastema differentiation. Additionally, we performed amputations to isolate fragments consisting of one, two, and three segments from the intestinal trunk region. We found that S. malaquini requires at least two to three segments to successfully regenerate the whole body. In addition, we verified a variable capacity to regenerate depending upon the gut region, with structures of the foregut greatly impairing some steps of the regenerative process. Our work notably addresses the gap in knowledge concerning gut formation and its impact on regenerative capabilities. Ongoing research is crucial to unravel the role of gut tissue specificity and plasticity during regeneration in annelids, and particularly in syllids.
期刊介绍:
Evolution & Development serves as a voice for the rapidly growing research community at the interface of evolutionary and developmental biology. The exciting re-integration of these two fields, after almost a century''s separation, holds much promise as the focus of a broader synthesis of biological thought. Evolution & Development publishes works that address the evolution/development interface from a diversity of angles. The journal welcomes papers from paleontologists, population biologists, developmental biologists, and molecular biologists, but also encourages submissions from professionals in other fields where relevant research is being carried out, from mathematics to the history and philosophy of science.