单细胞转录组综合分析发现了一种新型的与血统可塑性相关的癌细胞类型,它参与了前列腺癌的进展。

IF 9.7 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Faming Zhao, Tingting Zhang, Jinlan Wei, Liang Chen, Zaoqu Liu, Yang Jin, Mingsheng Liu, Hongqing Zhou, Yanxia Hu, Xia Sheng
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引用次数: 0

摘要

背景:癌细胞可塑性是指肿瘤细胞在微环境压力下改变自身特性并获得新的生物学特性的能力。在前列腺癌(PCa)中,细胞系可塑性通常会导致耐药性和向神经内分泌(NE)系的跨分化。然而,确定癌细胞是否具有与系可塑性相关的状态仍是一项挑战:方法:基于13个多中心人类PCa大样本转录组队列(样本数=3314)和9个PCa实验模型的大样本转录组数据集,我们建立了一个整合的品系可塑性相关基因特征,称为LPSig。利用这一基因特征,我们应用AUCell富集分析,从我们组建的一个全面的单细胞RNA测序(scRNA-seq)元图谱(包括10个公开的人类PCa scRNA-seq数据集(样本=93,细胞=222,529))中识别出了具有高度系谱可塑性的细胞群。此外,我们还采用了额外的人类 PCa scRNA-seq 数据集、人类 PCa 组织的多重免疫组化染色、体外和体内功能实验以及 qPCR 和 Western 印迹分析来验证我们的发现:我们发现,LPSig能精细捕捉PCa进展过程中肿瘤系可塑性的动态变化,准确估计系可塑性的状态。基于LPSig,我们从本研究收集的人类PCa scRNA-seq元图谱中发现了一个之前未定义的与品系可塑性相关的PCa细胞(LPCs)少数群体。此外,深入研究还发现了 LPCs 在 PCa 进展过程中的跨分化、肿瘤复发和患者生存率低下等方面的关键作用。此外,我们还发现 HMMR 是 LPCs 的代表性细胞表面标志物,并通过其他 scRNA-seq 数据集和多重免疫组化方法进行了验证。此外,HMMR受到雄激素受体(AR)的转录抑制,是侵袭性腺癌特征和NE表型所必需的:我们的研究发现了一种新的与细胞系可塑性相关的细胞群,它们具有低AR活性、干性特征和高HMMR表达,可能有助于PCa的不良预后:本研究得到了国家重点研发计划(2022YFA0807000)、国家自然科学基金(82160584)、昆明医科大学前列腺癌先进诊疗技术创新团队(CXTD202216)、云南省中青年学术带头人后备人才(202105AC160013)的资助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrated single-cell transcriptomic analyses identify a novel lineage plasticity-related cancer cell type involved in prostate cancer progression.

Background: Cancer cell plasticity is the ability of neoplastic cells to alter their identity and acquire new biological properties under microenvironmental pressures. In prostate cancer (PCa), lineage plasticity often results in therapy resistance and trans-differentiation to neuroendocrine (NE) lineage. However, identifying the cancer cells harboring lineage plasticity-related status remains challenging.

Methods: Based on 13 multi-center human PCa bulk transcriptomic cohorts (samples = 3314) and 9 bulk transcriptomic datasets derived from PCa experimental models, we established an integrated lineage plasticity-related gene signature, termed LPSig. Leveraging this gene signature, AUCell enrichment analysis was applied to identify the cell population with high lineage plasticity from a comprehensive single-cell RNA-sequencing (scRNA-seq) meta-atlas assembled by us, which consisted of 10 public human PCa scRNA-seq datasets (samples = 93, cells = 222,529). Moreover, additional scRNA-seq dataset of human PCa, multiplex immunohistochemistry staining for human PCa tissues, in vitro and in vivo functional experiments, as well as qPCR and Western blot analyses were employed to validate our findings.

Findings: We found that LPSig could finely capture the dynamics of tumor lineage plasticity throughout the progression of PCa, accurately estimating the status of lineage plasticity. Based on LPSig, we identified a previously undefined minority population of lineage plasticity-related PCa cells (LPCs) from the human PCa scRNA-seq meta-atlas assembled by this study. Furthermore, in-depth dissection revealed pivotal roles of LPCs in trans-differentiation, tumor recurrence, and poor patient survival during PCa progression. Furthermore, we identified HMMR as a representative cell surface marker for LPCs, which was validated using additional scRNA-seq datasets and multiplexed immunohistochemistry. Moreover, HMMR was transcriptionally inhibited by androgen receptor (AR), and was required for the aggressive adenocarcinoma features and NE phenotype.

Interpretation: Our study uncovers a novel population of lineage plasticity-related cells with low AR activity, stemness-like traits, and elevated HMMR expression, that may facilitate poor prognosis in PCa.

Funding: This work was supported by National Key R&D Program of China (2022YFA0807000), National Natural Science Foundation of China (82160584), Advanced Prostate Cancer Diagnosis and Treatment Technology Innovation Team of Kunming Medical University (CXTD202216), and Reserve Talents of Young and Middle-aged Academic Leaders in Yunnan Province (202105AC160013).

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来源期刊
EBioMedicine
EBioMedicine Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
17.70
自引率
0.90%
发文量
579
审稿时长
5 weeks
期刊介绍: eBioMedicine is a comprehensive biomedical research journal that covers a wide range of studies that are relevant to human health. Our focus is on original research that explores the fundamental factors influencing human health and disease, including the discovery of new therapeutic targets and treatments, the identification of biomarkers and diagnostic tools, and the investigation and modification of disease pathways and mechanisms. We welcome studies from any biomedical discipline that contribute to our understanding of disease and aim to improve human health.
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