中国出现含 blaOXA-181 的耐十环素克雷伯菌。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Yinfei Fang, Zhaoxia Wu, Wen Zheng, Hongwei Zhou, Lingna Hu, Qiong Xiao, Lihong Bo, Kai Shen, Yongjun Ma
{"title":"中国出现含 blaOXA-181 的耐十环素克雷伯菌。","authors":"Yinfei Fang, Zhaoxia Wu, Wen Zheng, Hongwei Zhou, Lingna Hu, Qiong Xiao, Lihong Bo, Kai Shen, Yongjun Ma","doi":"10.1007/s42770-024-01524-6","DOIUrl":null,"url":null,"abstract":"<p><p>We report the isolation of a bla<sub>OXA-181</sub>-positive, tigecycline-resistant Klebsiella aerogenes strain KA04 from a Chinese inpatient's fecal sample. Species identification was performed using MALDI-TOF MS. The antibiotic susceptibilities were assessed via the broth microdilution method. To elucidate the transmission and genetic structure of the bla<sub>OXA-181</sub> gene, conjugation assays and whole-genome sequencing (WGS) were performed. KA04 displayed resistance to carbapenems, quinolones, piperacillin/tazobactam and tigecycline. Through WGS and conjugation experiments, it was possible to confirm bla<sub>OXA-181</sub> and qnrS1 genes causing antibiotic resistance were located on a 51-kb IncX3 type mobile plasmid, bla<sub>OXA-181</sub> gene could be successfully transferred into E. coli EC600 at a conjugation frequency of 1.1 × 10<sup>- 4</sup>. tet(A) gene was located on both the chromosome and non-transmissible IncFIB(K) plasmid. This is a tigecycline-resistant K. aerogenes harboring bla<sub>OXA-181</sub> isolate from human fecal sample, highlighting a significant public health concern. Further comprehensive surveillance is needed.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Emergence of bla<sub>OXA-181</sub>-bearing tigecycline-resistant Klebsiella aerogenes in China.\",\"authors\":\"Yinfei Fang, Zhaoxia Wu, Wen Zheng, Hongwei Zhou, Lingna Hu, Qiong Xiao, Lihong Bo, Kai Shen, Yongjun Ma\",\"doi\":\"10.1007/s42770-024-01524-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We report the isolation of a bla<sub>OXA-181</sub>-positive, tigecycline-resistant Klebsiella aerogenes strain KA04 from a Chinese inpatient's fecal sample. Species identification was performed using MALDI-TOF MS. The antibiotic susceptibilities were assessed via the broth microdilution method. To elucidate the transmission and genetic structure of the bla<sub>OXA-181</sub> gene, conjugation assays and whole-genome sequencing (WGS) were performed. KA04 displayed resistance to carbapenems, quinolones, piperacillin/tazobactam and tigecycline. Through WGS and conjugation experiments, it was possible to confirm bla<sub>OXA-181</sub> and qnrS1 genes causing antibiotic resistance were located on a 51-kb IncX3 type mobile plasmid, bla<sub>OXA-181</sub> gene could be successfully transferred into E. coli EC600 at a conjugation frequency of 1.1 × 10<sup>- 4</sup>. tet(A) gene was located on both the chromosome and non-transmissible IncFIB(K) plasmid. This is a tigecycline-resistant K. aerogenes harboring bla<sub>OXA-181</sub> isolate from human fecal sample, highlighting a significant public health concern. Further comprehensive surveillance is needed.</p>\",\"PeriodicalId\":9090,\"journal\":{\"name\":\"Brazilian Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s42770-024-01524-6\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-024-01524-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

我们报告了从一名中国住院病人的粪便样本中分离出一株 blaOXA-181 阳性、耐虎头环素的产气克雷伯氏菌 KA04。采用 MALDI-TOF MS 进行了菌种鉴定。抗生素敏感性通过肉汤微稀释法进行评估。为阐明 blaOXA-181 基因的传播和遗传结构,进行了共轭测定和全基因组测序(WGS)。KA04 对碳青霉烯类、喹诺酮类、哌拉西林/他唑巴坦和替加环素具有耐药性。通过 WGS 和共轭实验,可以确认引起抗生素耐药性的 blaOXA-181 和 qnrS1 基因位于一个 51-kb 的 IncX3 型移动质粒上,blaOXA-181 基因可以以 1.1 × 10- 4 的共轭频率成功转入大肠杆菌 EC600。这是一个从人类粪便样本中分离出的对替加环素耐药的产气荚膜梭菌,携带 blaOXA-181,突显了一个重大的公共卫生问题。需要进一步进行全面监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Emergence of blaOXA-181-bearing tigecycline-resistant Klebsiella aerogenes in China.

We report the isolation of a blaOXA-181-positive, tigecycline-resistant Klebsiella aerogenes strain KA04 from a Chinese inpatient's fecal sample. Species identification was performed using MALDI-TOF MS. The antibiotic susceptibilities were assessed via the broth microdilution method. To elucidate the transmission and genetic structure of the blaOXA-181 gene, conjugation assays and whole-genome sequencing (WGS) were performed. KA04 displayed resistance to carbapenems, quinolones, piperacillin/tazobactam and tigecycline. Through WGS and conjugation experiments, it was possible to confirm blaOXA-181 and qnrS1 genes causing antibiotic resistance were located on a 51-kb IncX3 type mobile plasmid, blaOXA-181 gene could be successfully transferred into E. coli EC600 at a conjugation frequency of 1.1 × 10- 4. tet(A) gene was located on both the chromosome and non-transmissible IncFIB(K) plasmid. This is a tigecycline-resistant K. aerogenes harboring blaOXA-181 isolate from human fecal sample, highlighting a significant public health concern. Further comprehensive surveillance is needed.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信