模拟低温热修复对热带杂酚油污染土壤微生物群落的影响。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Daniel Di Pace Soares Penna, Valéria Maia Merzel, Juliana Gardenalli de Freitas, Kelly Johanna Hidalgo Martinez, Alexandre Muselli Barbosa, Cristina Rossi Nakayama
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引用次数: 0

摘要

在为多环芳烃(PAH)污染的土壤寻找更可持续的修复策略的过程中,热修复和生物修复(TEB)的综合应用可以通过将加热与生物降解联系起来,使用影响较小的温度。然而,人们对加热对土壤微生物群的影响仍然知之甚少,尤其是在热带地区的土壤中。这项研究调查了低温加热对杂酚油污染土壤细菌的影响。我们使用培养依赖法和 16 S rRNA 测序法比较了在微生态系统中加热至 60 oC 和 100 oC 1 小时的土壤样本的微生物群落。与对照土壤相比,加热至 60 °C 降低了可培养异养菌的密度(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of simulated low-temperature thermal remediation on the microbial community of a tropical creosote contaminated soil.

In the search for more sustainable remediation strategies for PAH-contaminated soils, an integrated application of thermal remediation and bioremediation (TEB) may allow the use of less impacting temperatures by associating heating to biological degradation. However, the influence of heating on soil microbiota remains poorly understood, especially in soils from tropical regions. This work investigated the effects of low-temperature heating on creosote-contaminated soil bacteria. We used culture-dependent and 16 S rRNA sequencing methods to compare the microbial community of soil samples heated to 60 and 100 oC for 1 h in microcosms. Heating to 60 °C reduced the density of cultivable heterotrophic bacteria compared to control soil (p < 0.05), and exposure to 100 °C inactivated the viable heterotrophic community. Burkholderia-Caballeronia-Paraburkholderia (BCP) group and Sphingobium were the predominant genera. Temperature and incubation time affected the Bray-Curtis dissimilarity index (p < 0.05). At 60 °C and 30 days incubation, the relative abundance of Sphingobium decreased and BCP increased dominance. The network of heated soil after 30 days of incubation showed fewer nodes and edges but maintained its density and complexity. Both main genera are associated with PAH degradation, suggesting functional redundancy and a likely potential of soil microbiota to maintain biodegradation ability after exposure to higher temperatures. We concluded that TEB can be considered as a potential strategy to bioremediate creosote-contaminated soils, allowing biodegradation in temperature ranges where thermal remediation does not completely remove contaminants. However, we recommend further research to determine degradation rates with this technology.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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