作为仔猪氧化应激潜在指标的生理、基因表达和行为。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Raúl David Guevara, Jose J Pastor, Sergi López-Vergé, Xavier Manteca, Gemma Tedo, Pol Llonch
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引用次数: 0

摘要

本研究的目的是开发一种猪模型,用于研究对仔猪福利负面影响较小的氧化应激。我们进行了四项独立试验(A、B、C 和 D),使用单次腹腔注射脂多糖(LPS)作为免疫挑战,旨在评估不同年龄的仔猪在健康动物体内引发可测量的急性氧化应激反应所需的最小 LPS 剂量。在试验 A 中,仔猪在断奶后 41 天(p.w.)接受 25 µg/KgBW 的 LPS 剂量。在试验 B 中,仔猪在断奶后 28 天注射 25 µg/KgBW 的 LPS;在试验 C 和 D 中,仔猪分别在断奶后 21 天注射 50 µg/KgBW 的 LPS。仔猪被随机分配到 T1(生理盐水对照组)或 T2(LPS 挑战组)。通过测定血浆和肠道组织中谷胱甘肽过氧化物酶(GPx)、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的酶活性来衡量氧化应激反应。评估了氧化应激和炎症标志物的肠道基因表达。还记录了动物的不适行为(喘气、匍匐、颤抖和呕吐)。在四次试验中,即使剂量和猪龄相似,血浆和肠道氧化应激反应也不一致,这可能是由于个体差异造成的。抗炎细胞因子(IL10)、氧化前体(iNOS)和抗氧化标志物(GPx4、MnSOD 和 CAT)的相对基因表达在 Ctrl 和 LPS 处理之间存在差异(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physiology, gene expression, and behavior as potential indicators of oxidative stress in piglets.

The goal of the current study was to develop a pig model to investigate oxidative stress with a low negative impact on piglet welfare. Four independent trials (A, B, C, and D) were performed using a single intraperitoneal shot of lipopolysaccharide (LPS) as an immune challenge, aiming to assess the minimal LPS dose for piglets of different age to trigger a measurable acute oxidative stress response in healthy animals. In trial A, piglets received an LPS dose of 25 µg/KgBW at 41 days post-weaning (p.w.). In trial B, piglets received 25 µg/KgBW of LPS at 28 days p.w., in trials C And D, piglets were injected with 50 µg/KgBW of LPS at 21 days p.w., respectively. Piglets were randomly allocated either to the T1) Control group with saline solution (Ctrl), or T2) LPS challenge (LPS). The oxidative stress response was measured through the enzymatic activity of glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT), in both plasma and intestinal tissues. Intestinal gene expression of oxidative stress and inflammatory markers was assessed. Discomfort behaviors (panting, prostration, trembling, and vomits) were also recorded. Plasmatic and intestinal oxidative stress response was inconsistent across the four trials even when the dose and pig age were similar, possibly due to individual variability. Relative gene expression differences of anti-inflammatory cytokines (IL10), oxidation precursor (iNOS), and antioxidant markers (GPx4, MnSOD, and CAT) were detected between Ctrl and LPS treatment (P < 0.05) when assessed. Behavioral observations were sensitive to the LPS dose relative to Ctrl (P < 0.05) in all four trials. These results suggest that behavioral observations can be used as a non-invasive methodology to detect the presence of oxidative stress in pigs in challenging conditions. Behavioral observations were more sensitive than other indicators (i.e., biomarkers and gene expression) in the current study. However, a sensitivity scale system needs to be developed to qualify and rank the impact of oxidative stress in pigs.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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