定量检测人脑脊液中丝氨酸 129 磷酸化 α-突触核蛋白的免疫测定法。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-15 DOI:10.1080/17576180.2024.2407718

Achani M Silva, Elizabeth S Hickford, Paul Cutler

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引用次数: 0

摘要

α-突触核蛋白（α-Syn）磷酸化与帕金森病发病机制之间的联系尚未完全阐明，部分原因是分析方法的特异性和灵敏度有限，导致有关该蛋白病理生理水平的数据相互矛盾。我们评估了用于定量 pSer129 α-Syn 的抗体，结果发现一种灵敏而特异的检测方法适用于帕金森病和对照组 CSF，而且两种人群之间没有发现显著差异。使用商业试剂盒测定了总α-Syn，结果表明总α-Syn与pSer129 α-Syn之间存在正相关。

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An immunoassay for the quantification of phosphorylated α-synuclein at serine 129 in human cerebrospinal fluid.

The link between alpha Synuclein (α-Syn) phosphorylation and Parkinson's disease pathogenesis has not been fully elucidated, in part due to analytical methods with finite specificity and sensitivity, resulting in conflicting data on pathophysiological levels of the protein.One factor hindering the assessment of the role of pSer129 α-Syn is the lack of a fit for purpose assay. Antibodies were assessed for quantification of pSer129 α-Syn, resulting in a sensitive and specific assay suitable for use in Parkinson's disease and control CSF, with no significant difference found between the two populations. Total α-Syn was measured using a commercial kit, demonstrating a positive correlation between total and pSer129 α-Syn.This adds to available methods for pSer129 α-Syn in support of α-synucleinopathy research.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.