组蛋白伴侣 ASF1 和 HIRA 是端粒长度和 45S rDNA 拷贝数平衡所必需的。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Adéla Machelová, Martina Nešpor Dadejová, Michal Franek, Guillaume Mougeot, Lauriane Simon, Samuel Le Goff, Céline Duc, Jasmin Bassler, Martin Demko, Jana Schwarzerová, Sophie Desset, Aline V. Probst, Martina Dvořáčková
{"title":"组蛋白伴侣 ASF1 和 HIRA 是端粒长度和 45S rDNA 拷贝数平衡所必需的。","authors":"Adéla Machelová,&nbsp;Martina Nešpor Dadejová,&nbsp;Michal Franek,&nbsp;Guillaume Mougeot,&nbsp;Lauriane Simon,&nbsp;Samuel Le Goff,&nbsp;Céline Duc,&nbsp;Jasmin Bassler,&nbsp;Martin Demko,&nbsp;Jana Schwarzerová,&nbsp;Sophie Desset,&nbsp;Aline V. Probst,&nbsp;Martina Dvořáčková","doi":"10.1111/tpj.17041","DOIUrl":null,"url":null,"abstract":"<p>Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and <i>45S rDNA</i> loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in <i>asf1a1b</i> and <i>hira</i> mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing <i>45S rRNA</i> genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase <i>in vivo</i>, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 3","pages":"1125-1141"},"PeriodicalIF":6.2000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17041","citationCount":"0","resultStr":"{\"title\":\"The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis\",\"authors\":\"Adéla Machelová,&nbsp;Martina Nešpor Dadejová,&nbsp;Michal Franek,&nbsp;Guillaume Mougeot,&nbsp;Lauriane Simon,&nbsp;Samuel Le Goff,&nbsp;Céline Duc,&nbsp;Jasmin Bassler,&nbsp;Martin Demko,&nbsp;Jana Schwarzerová,&nbsp;Sophie Desset,&nbsp;Aline V. Probst,&nbsp;Martina Dvořáčková\",\"doi\":\"10.1111/tpj.17041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and <i>45S rDNA</i> loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in <i>asf1a1b</i> and <i>hira</i> mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing <i>45S rRNA</i> genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase <i>in vivo</i>, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.</p>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"120 3\",\"pages\":\"1125-1141\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17041\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17041\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17041","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

染色质复合物在复制、转录和 DNA 修复过程中促进组蛋白从染色质中装载和排出,染色质复合物的精确协调对基因组稳定性有重大影响。在这项研究中,我们研究了拟南芥 H3 组蛋白伴侣素 ANTI-SILENCING FUNCTION 1(ASF1)和 HISTONE REGULATOR A(HIRA)在维护端粒和 45S rDNA 位点中的作用,这些基因组位点特别容易受到染色质结构变化的影响。我们发现 ASF1 和 HIRA 对端粒长度的调节都至关重要,因为 ASF1a1b 和 HIRA 突变体的端粒明显缩短。然而,这些较短的端粒仍然定位在核仁周围,并表现出与野生型相当的相对 H3 占有率。除了调节端粒长度,ASF1 和 HIRA 还有助于沉默 45S rRNA 基因并影响其拷贝数。此外,ASF1还支持全球异染色质的维持。我们的研究结果还表明,ASF1能瞬时结合体内的端粒重复结合1蛋白和端粒酶的N末端,这表明ASF1组蛋白伴侣与端粒维持机制之间存在物理联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and 45S rDNA loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in asf1a1b and hira mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing 45S rRNA genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase in vivo, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信