在水稻中,蔗糖通过与 K48 链接的多泛素化作用降解花抑制因子 Ghd7,从而诱导开花。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lae-Hyeon Cho, Jinmi Yoon, Gibeom Baek, Win Tun, Hyeok Chan Kwon, Dae-Woo Lee, Seok-Hyun Choi, Yang-Seok Lee, Jong-Seong Jeon, Gynheung An
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引用次数: 0

摘要

蔗糖在几种代谢途径和各种发育过程中发挥着信号分子的作用。然而,蔗糖调控这些过程的分子机制在很大程度上仍然未知。在本研究中,我们证明蔗糖通过介导抑制水稻开花的调控蛋白的稳定性来促进开花。外源施用蔗糖可通过诱导花源基因的表达来促进开花。通过基因修饰(如过表达液泡转化酶 OsVIN2 或突变蔗糖转运体 OsSUT2)降低韧皮部的蔗糖水平会延迟开花。对花期调控基因相对转录水平的分析表明,蔗糖激活了花源上游的 Ehd1,而对其他上游基因的表达没有显著影响。对蔗糖处理主要花序抑制因子后蛋白质稳定性的研究发现,Ghd7 蛋白被特异性降解。Ghd7蛋白与E3连接酶IPA INTERACTING PROTEIN1(IPI1)相互作用,蔗糖通过IPI1诱导Ghd7发生K48连接的多泛素化,导致蛋白降解。缺失 IPI1 的突变体会延迟开花,这证实了 IPI1 在调节参与开花的蛋白质中的作用。我们的结论是,蔗糖是通过 IPI1 促进 Ghd7 降解从而诱导开花的信号分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice.

Sucrose functions as a signaling molecule in several metabolic pathways as well as in various developmental processes. However, the molecular mechanisms by which sucrose regulates these processes remain largely unknown. In the present study, we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice. Exogenous application of sucrose promoted flowering by inducing florigen gene expression. Reduction of sucrose levels in the phloem through genetic modifications, such as the overexpression of the vacuolar invertase OsVIN2 or the mutation of OsSUT2, a sucrose transporter, delayed flowering. Analysis of relative transcript levels of floral regulatory genes showed that sucrose activated Ehd1 upstream of the florigen, with no significant effect on the expression of other upstream genes. Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded. The Ghd7 protein interacted with the E3 ligase IPA INTERACTING PROTEIN1 (IPI1), and sucrose-induced K48-linked polyubiquitination of Ghd7 via IPI1, leading to protein degradation. Mutants defective in IPI1 delayed flowering, confirming its role in modulating proteins involved in flowering. We conclude that sucrose acts as a signaling molecule to induce flowering by promoting Ghd7 degradation via IPI1.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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