在甜瓜-镰刀菌-FOM1.2相互作用病理系统中产生多基因抗性的基因标记。

IF 4.2 3区生物学 Q1 PLANT SCIENCES

Plant Biology Pub Date : 2025-01-01 Epub Date: 2024-10-15 DOI:10.1111/plb.13729

N Sadeghpour, H A Asadi-Gharneh, M Nasr-Esfahani, B Rahimiardkapan, A Nasr-Esfahani, A Nasr-Esfahani, M Monazah

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引用次数: 0

摘要

在任何育种计划中，开发具有抗Fusarium oxysporum f. sp. Melonis-(FOM) race1.2能力的甜瓜基因型都是一个主要目标。在本研究中，我们确定了 11 个基因标记在 FOM1.2 与甜瓜相互作用过程中对多基因抗性的作用。qRT-PCR 分析阐明了抗性基因 AMT、DXPR、Fom-2、GLUC、GalS、GRF3、MLO、PRK、RuBlsCo、TLP 和 WRKY 在接种后 7、14 和 21 天（dpi）在'Shante-F1'和'Khatouni'以及易感基因'Shante-T'和'Shahabadi'中的上调情况。我们还研究了防御相关酶活性的变化：瓜根中的几丁质酶（CHI）、β-1,3-葡聚糖酶（GLU）和过氧化物酶（POX）。在抗性 "Shante-F1 "和 "Shahabadi "的叶片和根部组织中，AMT、GLUC 和 DXPR 的转录物上调。在 7 dpi 时，'Shante-F1'根中 GalS 和 GRF3 的转录水平分别增加了 6.77 倍和 6.83 倍，而在 14 dpi 时，PRK、TLP 和 WRKYe 的转录水平分别增加了 7.84 倍、5.15 倍和 12.26 倍。然而，在 21 dpi 时，Fom-2 和 MLO 的转录水平分别增加了 5.18 倍和 8.46 倍。此外，抗性基因型叶片的 RBC 转录水平在 14 dpi 达到峰值，增加了 4.9 倍，而 AMT 在 21 dpi 增加了 2.94 倍，'Shante-F'的 GLUC 和 DXPR 在 14 dpi 分别增加了 7.11 倍和 2.91 倍。抗性品种的防御相关酶活性也上调了三倍。FOM1.2 诱导的甜瓜转录组的动态变化强调，抗性机制主要是通过涉及 CHI、GLU 和 POX 防御反应的信号通路来调节的。令人惊讶的是，AMT 基因基本上对霜霉病（Pseudoperonospora cubensis）有抗性；GLUC、MLO 和 PRK 对白粉病（Sphaerotheca fusca）有抗性；TLP 和 WRKY 对疫霉病（Phytophthora capsici）有抗性；GRF3 和 RBC 对根结线虫（Meloidogyne spp.这些基因在抗性基因型中上调，表明它们具有双重作用，可同时抵抗一种以上的病害。

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Gene markers generating polygenic resistance in melon-Fusarium wilt-FOM1.2 interaction pathosystem.

Developing melon genotypes with resistance to Fusarium oxysporum f. sp. Melonis-(FOM) race1.2 is a major goal in any breeding program. In this study, we identified the role of 11 gene markers that contribute to polygenic resistance during the FOM1.2-melon interaction. qRT-PCR analysis elucidated upregulation of candidate marker genes AMT, DXPR, Fom-2, GLUC, GalS, GRF3, MLO, PRK, RuBlsCo, TLP and WRKY in resistant 'Shante-F1' and 'Khatouni', and susceptible 'Shante-T' and 'Shahabadi' at 7, 14 and 21 days post-inoculation (dpi). We also studied changes in defence-related enzyme activity: chitinase (CHI), β-1,3-glucanase (GLU) and peroxidase (POX) in melon roots. AMT, GLUC and DXPR transcripts were upregulatied in leaf and root tissues of the resistant 'Shante-F1' and 'Shahabadi'. Transcript levels for GalS and GRF3 increased 6.77- and 6.83-fold in roots of 'Shante-F1' at 7 dpi, whereas in PRK, TLP and WRKY theye increased by 7.84-, 5.15- and 12.26-fold at 14 dpi, respectively. However, transcript levels increased by 5.18-fold for Fom-2 and 8.46-fold for MLO at 21 dpi. Also, RBC transcript level peaked at 14 dpi with 4.9-fold increase in leaves of resistant genotypes, whereas AMT increased 2.94-fold at 21 dpi, and GLUC and DXPR increased 7.11- and 2.91-fold at 14 dpi in 'Shante-F', respectively. Defence-related-enzyme activity was also upregulated three-fold in resistant varieties. The dynamic shifts in the melon transcriptome induced by FOM1.2 emphasize that resistance mechanisms are predominantly regulated through signalling pathways involving CHI, GLU, and POX defence response. Surprisingly, the AMT gene, basically resistant to downy mildew, Pseudoperonospora cubensis; GLUC, MLO and PRK resistant to powdery mildew (Sphaerotheca fusca); TLP and WRKY resistant to Phytophthora blight (Phytophthora capsici); and GRF3 and RBC resistant to root knot nematodes (Meloidogyne spp.) were upregulated in resistant genotypes, indicating a dual role of these genes in resistance to more than one disease at a time.

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来源期刊

Plant Biology 生物-植物科学

CiteScore

8.20

自引率

2.60%

发文量

109

审稿时长

3 months

期刊介绍： Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.