冠醚基人工 K+ 选择性离子过滤器

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

ChemBioChem Pub Date : 2025-01-02 Epub Date: 2024-11-09 DOI:10.1002/cbic.202400789

Pengyang Xin, Zhihui Jiu, Linlin Shi, Yuqing Shu, Yufei Song, Yonghui Sun

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引用次数: 0

摘要

本研究通过单击反应成功合成了双（胆固醇-二苯并-18-冠-6-醚）-pillar[5]arene化合物1，由于其膜锚胆固醇基团可自发插入脂质双层膜形成离子通道，对K+的转运活性显著优于Na+，K+/Na+的渗透比为4.58。化合物 1 的两个冠醚模块起到了类似于天然 K+ 通道的选择性过滤器的作用，通过约伯图和核磁共振滴定法确定它们与 K+ 的结合比例为 1:2。这种结构明确的单分子人工通道为构建高 K+/Na+ 选择性分子过滤器提供了思路。

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Crown-Ether-Based Artificial K⁺ Selective Ionic Filter.

In this study, we have successfully synthesized bis (cholesterol-dibenzo-18-crown-6-ether)-pillar[5]arene compound 1 through a click reaction, which could spontaneously insert into lipid bilayers to form ion channel due to the membrane anchor cholesterol group and show significant transport activity of K⁺ superior to Na⁺, with a permeability ratio of K⁺/Na⁺ equal to 4.58. Compound 1 two crown ether modules act as selective filters similar to natural K⁺ channel, which are determined to 1 : 2 binding stoichiometry to K⁺ by Job's plot and NMR titration. This structurally unambiguously unimolecule artificial channel provides ideas for constructing highly K⁺/Na⁺ selective molecular filters.

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来源期刊

ChemBioChem 生物-生化与分子生物学

CiteScore

6.10

自引率

3.10%

发文量

407

审稿时长

1 months

期刊介绍： ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).