{"title":"一种基于希夫碱结构的简单关断荧光化学传感器,用于超快、高选择性地痕量检测水溶液中的 Cu2+ 离子。","authors":"Jing Wang, Lu Ren, Qiang Sun, Yanqi Liu, Wanru Jia, Huihong Zhang, Dawei Zhang","doi":"10.1039/d4ay01649a","DOIUrl":null,"url":null,"abstract":"<p><p>A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu<sup>2+</sup>. The bright green fluorescence of DHP is quenched after the addition of Cu<sup>2+</sup>. The probe DHP exhibits good anti-interference performance against Cu<sup>2+</sup> in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu<sup>2+</sup> concentration at 0-5 μM, and it has a detection limit of 0.29 nM (<i>R</i><sup>2</sup> = 0.9949). The complexation ratio of the probe DHP to Cu<sup>2+</sup> is 2 : 1 and the complexation constant is 3.44 × 10<sup>4</sup> M<sup>-1</sup> (<i>R</i><sup>2</sup> = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu<sup>2+</sup> can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for <i>in vivo</i> cell imaging.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A simple turn-off fluorescent chemosensor based on a Schiff base structure for ultrafast and highly selective trace detection of Cu<sup>2+</sup> ions in aqueous solutions.\",\"authors\":\"Jing Wang, Lu Ren, Qiang Sun, Yanqi Liu, Wanru Jia, Huihong Zhang, Dawei Zhang\",\"doi\":\"10.1039/d4ay01649a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu<sup>2+</sup>. The bright green fluorescence of DHP is quenched after the addition of Cu<sup>2+</sup>. The probe DHP exhibits good anti-interference performance against Cu<sup>2+</sup> in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu<sup>2+</sup> concentration at 0-5 μM, and it has a detection limit of 0.29 nM (<i>R</i><sup>2</sup> = 0.9949). The complexation ratio of the probe DHP to Cu<sup>2+</sup> is 2 : 1 and the complexation constant is 3.44 × 10<sup>4</sup> M<sup>-1</sup> (<i>R</i><sup>2</sup> = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu<sup>2+</sup> can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for <i>in vivo</i> cell imaging.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-10-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4ay01649a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01649a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A simple turn-off fluorescent chemosensor based on a Schiff base structure for ultrafast and highly selective trace detection of Cu2+ ions in aqueous solutions.
A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu2+. The bright green fluorescence of DHP is quenched after the addition of Cu2+. The probe DHP exhibits good anti-interference performance against Cu2+ in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu2+ concentration at 0-5 μM, and it has a detection limit of 0.29 nM (R2 = 0.9949). The complexation ratio of the probe DHP to Cu2+ is 2 : 1 and the complexation constant is 3.44 × 104 M-1 (R2 = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu2+ can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for in vivo cell imaging.