一种基于希夫碱结构的简单关断荧光化学传感器,用于超快、高选择性地痕量检测水溶液中的 Cu2+ 离子。

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Jing Wang, Lu Ren, Qiang Sun, Yanqi Liu, Wanru Jia, Huihong Zhang, Dawei Zhang
{"title":"一种基于希夫碱结构的简单关断荧光化学传感器,用于超快、高选择性地痕量检测水溶液中的 Cu2+ 离子。","authors":"Jing Wang, Lu Ren, Qiang Sun, Yanqi Liu, Wanru Jia, Huihong Zhang, Dawei Zhang","doi":"10.1039/d4ay01649a","DOIUrl":null,"url":null,"abstract":"<p><p>A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu<sup>2+</sup>. The bright green fluorescence of DHP is quenched after the addition of Cu<sup>2+</sup>. The probe DHP exhibits good anti-interference performance against Cu<sup>2+</sup> in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu<sup>2+</sup> concentration at 0-5 μM, and it has a detection limit of 0.29 nM (<i>R</i><sup>2</sup> = 0.9949). The complexation ratio of the probe DHP to Cu<sup>2+</sup> is 2 : 1 and the complexation constant is 3.44 × 10<sup>4</sup> M<sup>-1</sup> (<i>R</i><sup>2</sup> = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu<sup>2+</sup> can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for <i>in vivo</i> cell imaging.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A simple turn-off fluorescent chemosensor based on a Schiff base structure for ultrafast and highly selective trace detection of Cu<sup>2+</sup> ions in aqueous solutions.\",\"authors\":\"Jing Wang, Lu Ren, Qiang Sun, Yanqi Liu, Wanru Jia, Huihong Zhang, Dawei Zhang\",\"doi\":\"10.1039/d4ay01649a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu<sup>2+</sup>. The bright green fluorescence of DHP is quenched after the addition of Cu<sup>2+</sup>. The probe DHP exhibits good anti-interference performance against Cu<sup>2+</sup> in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu<sup>2+</sup> concentration at 0-5 μM, and it has a detection limit of 0.29 nM (<i>R</i><sup>2</sup> = 0.9949). The complexation ratio of the probe DHP to Cu<sup>2+</sup> is 2 : 1 and the complexation constant is 3.44 × 10<sup>4</sup> M<sup>-1</sup> (<i>R</i><sup>2</sup> = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu<sup>2+</sup> can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for <i>in vivo</i> cell imaging.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-10-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4ay01649a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01649a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

设计并合成了一种简单的熄灭荧光探针--5-(二乙基氨基)-2-(肼甲基)苯酚(DHP),用于灵敏、选择性地检测 Cu2+。加入 Cu2+ 后,DHP 的亮绿色荧光被淬灭。在多种金属离子存在的情况下,探针 DHP 对 Cu2+ 具有良好的抗干扰性能。在 522 纳米波长处,DHP(10 μM)的荧光强度与 0-5 μM 的 Cu2+ 浓度呈良好的线性关系,其检测限为 0.29 nM(R2 = 0.9949)。探针 DHP 与 Cu2+ 的络合比为 2 :1 ,络合常数为 3.44 × 104 M-1 (R2 = 0.9974)。此外,探针 DHP 可使用 EDTA 回收,Cu2+ 可在 pH 值为 5-11 的条件下有效监测,在滴定管实验和实际水样中效果良好。HepG-2 细胞暴露于 DHP(50 μM)24 小时后,其存活率仍超过 90%,这表明 DHP 的毒性极低,可用于体内细胞成像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A simple turn-off fluorescent chemosensor based on a Schiff base structure for ultrafast and highly selective trace detection of Cu2+ ions in aqueous solutions.

A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu2+. The bright green fluorescence of DHP is quenched after the addition of Cu2+. The probe DHP exhibits good anti-interference performance against Cu2+ in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu2+ concentration at 0-5 μM, and it has a detection limit of 0.29 nM (R2 = 0.9949). The complexation ratio of the probe DHP to Cu2+ is 2 : 1 and the complexation constant is 3.44 × 104 M-1 (R2 = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu2+ can be effectively monitored at pH 5-11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for in vivo cell imaging.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信