预测小分子分配

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2024-10-22 DOI:10.1038/s41589-024-01774-3

Russell Johnson

引用次数: 0

摘要

生物分子凝聚物通过排斥或富集分子来实现其细胞功能。分析凝聚态的组成对于了解其特性和功能非常重要。在了解凝聚态的核酸和蛋白质成分方面已经做了大量工作；然而，人们对小分子的分区以及在凝聚态形成的条件下决定其富集或排斥的因素却知之甚少。现在，Thody 等人测量了大约 1700 种生物相关小分子在 4 种不同凝聚物中的分配情况，这些凝聚物是由不相关的蛋白质和蛋白质-DNA 混合物相分离形成的。为了确保他们分析的是各种结构的分配情况，研究小组使用了一个不同的代谢物和药物库，这些代谢物和药物并不是用一个共同的分子核心构建的。为了精确测量分配系数，研究小组开发了两种检测方法--一种基于离心分离，然后进行质谱分析；另一种基于荧光分析。这些结果表明，小分子的分配系数相差近六个数量级，但不同凝聚物之间却有相似之处，这表明由不同组成的大分子形成的凝聚物产生了物理上相似的内部环境。

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Predicting small-molecule partitioning

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.