用于治疗溃疡性结肠炎的益生元和益生菌双重递送的生物启发核壳微粒。

IF 10.5 1区 医学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Mengyao Liu , Shuang Ma , Jinglei Zhi , Mingming Wang , Ying Xu , Young-Rok Kim , Ke Luo
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引用次数: 0

摘要

植物乳杆菌(LP)是一种众所周知的益生菌株,对预防溃疡性结肠炎有益。然而,由于植物乳杆菌易受高度复杂的肠道菌群生态系统的影响,向结肠输送足够数量的植物乳杆菌仍然面临挑战。在此,我们介绍了一种离心机驱动的微喷嘴系统,该系统设计用于双层核壳藻酸盐微胶囊(DAM),可作为抗性淀粉纳米颗粒(RSNP,益生元)和LP(益生菌)双重递送的有效载体,用于治疗结肠炎。该系统可将 LP 和 RSNP 分别精确地装载在 DAM 的核心和外壳区域。结果表明,LP/RS@DAM 对 LP 的封装效率很高(每颗珠子可封装 108 个 CFU),RSNP 在外壳中的密集分布可有效保护 LP 不受酸性条件(pH 值为 2)的影响,即使在长期储存 30 天后,细胞存活率仍可保持在 52%。此外,在葡聚糖硫酸钠(DSS)诱导的结肠炎中,LP/RS@DAM 能有效促进短链脂肪酸的产生,从而减少炎症细胞因子,恢复肠道微生物多样性。我们相信,这种创新方法将为改善结肠炎管理提供潜在的解决方案,并为胃肠道疾病的定制治疗干预铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bioinspired core-shell microparticle for dual-delivery of prebiotic and probiotic for the treatment of ulcerative colitis

Bioinspired core-shell microparticle for dual-delivery of prebiotic and probiotic for the treatment of ulcerative colitis
Lactiplantibacillus plantarum (LP) is a well-known probiotic strain that has a beneficial effect in preventing ulcerative colitis. However, delivering a sufficient number of viable LP to the colon still face challenges due to its vulnerability to the highly complex intestinal flora ecosystem. Herein, we present a centrifuge-driven micronozzle system designed for double-layered core-shell alginate microcapsules (DAM), which can serve as an effective carrier for dual delivery of resistant starch nanoparticles (RSNP, prebiotic) and LP (probiotics) for the treatment of colitis. This system enables precise loading of LP and RSNP within the core and shell regions of DAM, respectively. The resulting LP/RS@DAM exhibited a high encapsulation efficiency of LP (108 CFU per bead), in which the dense distribution of RSNP in the shell effectively protected LP against acidic conditions (pH 2) and maintained the cell viability up to 52 % even after long-term storage for 30 days. Furthermore, LP/RS@DAM effectively enhances the production of short-chain fatty acids, leading to a reduction in inflammatory cytokines and restoration of intestinal microbial diversity in dextran sulfate sodium (DSS)-induced colitis. We believe that this innovative approach would offer a potential solution for improving colitis management and paving the way for tailored therapeutic interventions in gastrointestinal disorders.
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来源期刊
Journal of Controlled Release
Journal of Controlled Release 医学-化学综合
CiteScore
18.50
自引率
5.60%
发文量
700
审稿时长
39 days
期刊介绍: The Journal of Controlled Release (JCR) proudly serves as the Official Journal of the Controlled Release Society and the Japan Society of Drug Delivery System. Dedicated to the broad field of delivery science and technology, JCR publishes high-quality research articles covering drug delivery systems and all facets of formulations. This includes the physicochemical and biological properties of drugs, design and characterization of dosage forms, release mechanisms, in vivo testing, and formulation research and development across pharmaceutical, diagnostic, agricultural, environmental, cosmetic, and food industries. Priority is given to manuscripts that contribute to the fundamental understanding of principles or demonstrate the advantages of novel technologies in terms of safety and efficacy over current clinical standards. JCR strives to be a leading platform for advancements in delivery science and technology.
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