抗氧化肽 CHCIWM 和 CHCPWM 的性能研究与应用

IF 2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Liang Dong, Jun Ma, Yanan Li, Prof. Hailin Cong, Prof. Bing Yu
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引用次数: 0

摘要

当体内活性氧(ROS)水平异常升高时,会损害组织细胞,与各种慢性疾病的发生密切相关。抗氧化肽(APs)是一类具有抗氧化能力的活性短肽,具有重要的研究价值。本文根据APs的结构特点,设计合成了两种APs:CHIWM(AP1)和CHCPWM(AP2)。采用量子化学密度泛函理论(DFT)方法分析了 APs 的反应位点和活性。活性位点均位于色氨酸残基的吲哚杂环上,其中 AP1 的能隙和单点能较小。多种自由基清除试验表明,AP1 和 AP2 比阳性对照谷胱甘肽(GSH)具有更强的抗氧化活性。清除 DPPH 自由基的 IC50 值分别为 0.613 毫克/毫升和 0.606 毫克/毫升。在细胞实验中,AP1 和 AP2 都能清除细胞中的 ROS,减轻细胞损伤。总之,本文设计的 AP1 和 AP2 具有良好的抗氧化活性,有望应用于治疗由细胞氧化损伤引起的慢性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Performance Study and Application of Antioxidant Peptides CHCIWM and CHCPWM

Performance Study and Application of Antioxidant Peptides CHCIWM and CHCPWM

When the level of reactive oxygen species (ROS) increases abnormally in the body, it damages tissue cells and is closely related to the occurrence of various chronic diseases. Antioxidant peptides (APs) are a class of active short peptides with antioxidant capacity, which have great research value. In this paper, two APs were designed and synthesized based on the structural features of APs: CHIWM (AP1) and CHCPWM (AP2). The reactive sites and activities of APs were analyzed using the quantum chemical density functional theory (DFT) method. The reactive sites are all located in the indolyl heterocycle of tryptophan residues, among which AP1 has a smaller energy gap and single point energy. Multiple free radical scavenging assays showed that AP1 and AP2 had stronger antioxidant activity than the positive control, glutathione (GSH). For scavenging DPPH free radicals, the IC50s values were 0.613 mg/mL and 0.606 mg/mL, respectively. In cellular assays, both AP1 and AP2 were able to scavenge ROS in the cells and attenuate cellular damage. In conclusion, AP1 and AP2 designed in this paper have good antioxidant activity and have the potential to be applied in the treatment of chronic diseases caused by cellular oxidative damage.

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来源期刊
ChemistrySelect
ChemistrySelect Chemistry-General Chemistry
CiteScore
3.30
自引率
4.80%
发文量
1809
审稿时长
1.6 months
期刊介绍: ChemistrySelect is the latest journal from ChemPubSoc Europe and Wiley-VCH. It offers researchers a quality society-owned journal in which to publish their work in all areas of chemistry. Manuscripts are evaluated by active researchers to ensure they add meaningfully to the scientific literature, and those accepted are processed quickly to ensure rapid online publication.
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