分析药物与腐植酸之间的相互作用:利用夹带和高性能亲和微柱法进行表征

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
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引用次数: 0

摘要

为了促进全球水资源的可持续发展,水的再利用和循环利用日益增多,因此环境中作为微污染物存在的药物已成为人们特别关注的问题。药物通常会与腐殖酸等可溶性溶解有机物发生可逆的相互作用,这可能是决定这些化合物在环境中的生物利用度和影响的重要因素。在本研究中,使用了含有非共价夹带和固定腐植酸的高性能亲和微柱来检测这种制剂与四环素、卡马西平、环丙沙星和诺氟沙星的结合强度和相互作用。用奥尔德里奇腐植酸测得的结合常数与文献报道的数值非常吻合。此外,还利用腐植酸微柱研究了温度、离子强度和 pH 值对这些相互作用的影响。这种技术可以确定静电相互作用与非极性相互作用或氢键在这些结合过程中的相对重要性。这项研究说明了如何利用亲和微柱来筛选和统一量化药物与腐植酸的结合,以及研究这些相互作用的机制,通常只需少量结合剂(每个微柱 10 毫克,可用于 200-300 次实验),在几分钟内就能获得这些信息。使用夹带和亲和微柱可以支持对水和环境中的腐植酸或其他结合剂与其他多种微污染物进行类似的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of interactions between pharmaceuticals and humic acid: Characterization using entrapment and high-performance affinity microcolumns
The presence of pharmaceuticals as microcontaminants in the environment has become of particular concern given the growing increase in water reuse and recycling to promote global sustainability of this resource. Pharmaceuticals can often undergo reversible interactions with soluble dissolved organic material such as humic acid, which may be an important factor in determining the bioavailability and effects of these compounds in the environment. In this study, high-performance affinity microcolumns containing non-covalently entrapped and immobilized humic acid are used to examine the binding strength and interactions of this agent for tetracycline, carbamazepine, ciprofloxacin, and norfloxacin, all common pharmaceutical microcontaminants known to bind humic acid. The binding constants, as measured with Aldrich humic acid, have good agreement with values reported in the literature. In addition, the effects of temperature, ionic strength, and pH on these interactions are examined with the humic acid microcolumns. This technique makes it possible to determine the relative importance of electrostatic interactions vs non-polar interactions or hydrogen bonding on these binding processes. This study illustrates how affinity microcolumns can be used to screen and uniformly quantify binding by pharmaceuticals with humic acid, as well as to study the mechanisms of these interactions, with this information often being acquired in minutes and with small amounts of binding agent (∼10 mg per microcolumn, which could be used over 200–300 experiments). Use of entrapment and affinity microcolumns can support similar research for a wide range of other microcontaminants with humic acid or alternative binding agents found in water and the environment.
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来源期刊
Journal of Chromatography A
Journal of Chromatography A 化学-分析化学
CiteScore
7.90
自引率
14.60%
发文量
742
审稿时长
45 days
期刊介绍: The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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