R2R3-MYB 转录因子 GmMYB68 参与大豆异黄酮的积累

IF 6.1 2区 生物学 Q1 PLANT SCIENCES
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引用次数: 0

摘要

我们的目的是研究大豆转录因子 R2R3-MYB(GmMYB68)在异黄酮生物合成中的调控功能。通过全面的亚细胞和染色体定位分析,我们发现 GmMYB68 主要定位在细胞核中,并映射到染色体 Gm04 上。值得注意的是,该基因附近的 SSR 标记与种子异黄酮含量有显著相关性。GmMYB68 的过表达明显增加了异黄酮的含量,证实了它在调节异黄酮合成中的积极作用。GmMYB68 在大豆对非生物胁迫的响应中也起着至关重要的作用。利用 RNA-seq 和酵母单杂交技术,我们发现了 GmMYB68 与关键的异黄酮生物合成基因 GmCHS7 和 GmCHS8 之间错综复杂的相互作用。这些发现为异黄酮的生物合成机制提供了新的见解。此外,通过酵母双杂交实验,我们发现了与 GmMYB68 相互作用的蛋白质,这表明它们在生理活性化合物的合成和非生物胁迫响应中发挥作用。我们不仅阐明了 GmMYB68 在异黄酮生物合成和非生物胁迫响应中的调控机制,还构建了一个包括 GmMYB68、GmCHS7 和 GmCHS8 的分子网络。该网络为更好地理解大豆异黄酮的生物合成和改进大豆异黄酮生物合成的策略提供了理论基础。
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R2R3-MYB transcription factor GmMYB68 is involved in the accumulation of soybean isoflavones
We aimed to investigate the regulatory function of the soybean transcription factor R2R3-MYB (GmMYB68) in isoflavone biosynthesis. Through comprehensive subcellular and chromosomal localization analyses, we found that GmMYB68 was predominantly localized to the nucleus and mapped to chromosome Gm04. Notably, SSR markers near this gene significantly correlated with seed isoflavone content. GmMYB68 overexpression markedly increased isoflavone contents, confirming its positive role in regulating isoflavone synthesis. GmMYB68 also played a crucial role in the response of soybean to abiotic stress. Using RNA-seq and yeast one-hybrid techniques, we discovered an intricate interaction between GmMYB68 and key isoflavone biosynthesis genes GmCHS7 and GmCHS8. These findings provide novel insights into the mechanisms underlying isoflavone biosynthesis. Furthermore, using yeast two-hybrid experiments, we identified proteins interacting with GmMYB68, suggesting roles in the synthesis of physiologically active compounds and abiotic stress response. We not only elucidated the regulatory mechanisms of GmMYB68 in isoflavone biosynthesis and abiotic stress response but also constructed a molecular network encompassing GmMYB68, GmCHS7, and GmCHS8. This network provides a theoretical basis for a better understanding of and strategies for improving soybean isoflavone biosynthesis.
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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