TgCWIN2 介导的光同化物变化在调节香榧种子早期发育中的作用和机制

IF 6.1 2区 生物学 Q1 PLANT SCIENCES
Jinwei Suo , Jiayue Zhong , Minmin Yang, Qianxi Li, Yuanyuan Hu, Weiwu Yu, Jingwei Yan, Jiasheng Wu
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引用次数: 0

摘要

种子的早期发育对植物的繁殖至关重要,但对于像大香榧这样种子早期发育正常率较低的裸子植物来说,其背后的发育过程还不甚了解。为了填补这一知识空白,我们对大叶香榧种子发育的形态学和物候学进行了全面调查。利用 13C 标记分析,并结合摘叶和疏种处理,我们观察到光同化物的含量大幅增加,疏种处理下的蔗糖含量增加了近 10%,从而导致正常早期发育种子的比例增加,达到 15%。同时,通过整合多组学分析和瞬时过表达验证,我们发现了细胞壁转化酶编码基因 TgCWIN2,它在鹅掌楸种子早期发育过程中的蔗糖裂解中起着关键作用。进一步的基因共表达、双荧光素酶检测和酵母单杂交检测发现,TgWRKY31是TgCWIN2的候选调控因子,通过直接与TgCWIN2启动子结合对其表达产生积极影响。值得注意的是,TgWRKY31的瞬时过表达大大提高了TgCWIN2的表达,从而使早期发育正常的种子比例更高。我们的研究结果不仅让人们全面了解了鹅掌楸种子早期发育的基本机制,而且对于制定加强种子早期发育和提高产量的策略也至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds
Early seed development is vital for plant reproduction, but the processes behind this in gymnosperms like Torreya grandis, which has a low rate of normal early-developed seeds, are not well understood. To fill this knowledge gap, we embarked on a comprehensive investigation encompassing the morphology and phenology of seed development in T. grandis. Using the 13C labelling analysis, coupled with leaf removal and seed thinning treatments, we observed a substantial increase in the content of photo-assimilate, an almost 10% increase in sucrose content under seed thinning treatments, thereby leading to an increase in the proportion of normal early-developed seeds, reaching 15%. Concurrently, through the integration of multi-omics analyses and transient overexpression validation, we identified cell wall invertase coding gene, TgCWIN2, which plays a pivotal role in sucrose cleavage during the early development of T. grandis seeds. Further gene co-expression, dual-luciferase assay, and yeast one-hybrid assay revealed that TgWRKY31 was a candidate regulator of TgCWIN2, positively influencing its expression by direct binding to the TgCWIN2 promoter. Notably, TgWRKY31 transient overexpression substantially enhances the expression of TgCWIN2, thereby contributing to a higher proportion of normal early-developed seeds. Our findings not only provide a comprehensive understanding of the underlying mechanisms governing the early development of T. grandis seeds, but are also essential for establishing strategies to enhance early seed development and improve yield.
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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