通过改善珍珠龙胆石斑鱼（Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂）的免疫耐受谱，膳食红豆杉乙醇提取物可缓解豆粕诱导的肠炎

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-10-01 DOI:10.1016/j.cbd.2024.101335

Xin Lu, Yingrui Wu, Yinhui Peng, Kianann Tan, Ming Liu, Xueying Liang, Peng Xu, Mingzhong Liang, Wenjie Liu, Qingfang Gong, Xiaohui Cai

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引用次数: 0

摘要

水产养殖业缺乏针对豆粕诱发的肉食性鱼类肠炎（SBMIE）的保护措施。我们的初步研究发现，Lithospermum erythrorhizon（LE）乙醇提取物在改善 SBMIE 方面具有潜在的应用价值。我们进行了一项为期八周的喂养试验，研究乙醇提取物对珍珠龙胆石斑鱼SBMIE的保护作用，以明确乙醇提取物对免疫耐受谱的影响。对 360 尾珍珠龙胆石斑鱼采用了三种不同的饮食方案：1) 100 % 鱼粉（FM）；2) 50 % 鱼粉蛋白的豆粕替代物（SBM）；3) SBM 日粮 +0.2 % LE 乙醇提取物（SBMLE）。每种处理包括三个 1000 L 的蓄水池，每个蓄水池养 30 条鱼。生长性能结果表明，SBM 组的 WGR 和 SGR 显著下降（P < 0.05），而 FM 组和 SBMLE 组之间没有显著差异。三组的存活率无明显差异。结果表明，SBM喂养的鱼表现出肠炎，表现为粘膜皱褶缩短、固有膜增宽、血清免疫标志物（IgM、C3和C4）降低、促炎细胞因子（il17和il12）和免疫相关基因（tlr3和tlr9）表达上调。在 SBM 日粮中添加 0.2 % LE 乙醇提取物后，上述症状得到逆转，抗炎细胞因子（tgf-β1）基因表达显著增加（P < 0.05）。肠道转录组分析显示，FM组与SBM组之间的DEGs主要富集于FoxO信号通路，而SBM组与SBMLE组之间的DEGs则富集于MAPK信号通路和FoxO信号通路。RT-qPCR 结果还显示了 MAPK/FoxO 信号通路相关基因的变化，包括 Dusp1、jund、Irs2b、fbxo32 和 ccng2。总之，红豆杉乙醇提取物可通过调节MAPK/FoxO信号通路缓解SBMIE，有利于提高珍珠龙胆石斑鱼对日粮豆粕的免疫耐受和利用效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Dietary Lithospermum erythrorhizon ethanol extract alleviates soybean meal-induced enteritis by improving immune tolerance profile of pearl gentian grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

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The aquaculture industry has a shortage of objected protection against soybean meal-induced enteritis (SBMIE) in carnivorous fish caused by soybean meal feed. Our initial study discovered that Lithospermum erythrorhizon (LE) ethanol extract has potential application value in improving SBMIE. A feeding trial (for eight weeks) was conducted to investigate LE ethanol extract on pearl gentian grouper SBMIE of protection to clarify the influence of LE ethanol extract on the immune tolerance profile. Three hundred and sixty pearl gentian groupers were administered one of three distinct dietary regimes: 1) 100 % fish meal (FM); 2) soybean meal substitution of 50 % fish meal protein (SBM); 3) SBM diet +0.2 % LE ethanol extract (SBMLE). Each treatment included three 1000 L cisterns—each of cisterns with 30 fish. The preliminary weight of the fish varied between 72.01 g and 72.50 g. Growth performance results showed that WGR and SGR were significantly decreased in the SBM group (P < 0.05), while there was no significant difference between the FM and SBMLE groups. There was no significant difference in survival among the three groups. The results showed that SBM-fed fish exhibited enteritis manifested by mucosal fold shortening, lamina propria widening, decreased serum immune markers (IgM, C3, and C4), and up-regulated expression of pro-inflammatory cytokines (il17 and il12) and immune-related gene (tlr3, and tlr9). The addition of 0.2 % LE ethanol extract to the SBM diet, reversed the above symptoms, and anti-inflammatory cytokine (tgf-β1), gene expression increased significantly (P < 0.05). Intestinal transcriptome analysis exhibited that the DEGs between the FM group and the SBM group were mainly enriched in FoxO signaling pathway, while the DEGs between the SBM group and the SBMLE group were enriched in MAPK signaling pathway and FoxO signaling pathway. The RT-qPCR results also revealed changes in MAPK/FoxO signaling pathway-related genes, including Dusp1, jund, Irs2b, fbxo32, and ccng2. Overall, Lithospermum erythrorhizon ethanol extract may alleviate SBMIE by regulating MAPK/FoxO signaling pathway, which would be beneficial for enhancing the immune tolerance and utilization efficiency of pearl gentian groupers to dietary soybean meal.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464