{"title":"为圆筒形丝瓜（Luffa cylindrica (L.) Roem）开发高效、可重复的体外再生和转化协议","authors":"Zhengjing Wu, Chenfang Li, Yidian Gao, Hao Zhang, Yuan Long, Bingjie An, Sujuan Liu, Jingjing Wu","doi":"10.1016/j.sajb.2024.09.067","DOIUrl":null,"url":null,"abstract":"<div><div>To establish an efficient in vitro regeneration system and lay a foundation for further research, the effects of shelling, plant hormones, explant position, infection parameters and other factors on the in vitro regeneration of luffa cotyledons were studied. The results showed that the best medium for inducing adventitious buds from luffa cotyledons was Murashige and Skoog (MS) + Thidiazuron (TDZ) 0.3 mg·<em>L</em><sup>−1</sup>+ Indole-3-Butyric acid (IBA) 0.02 mg·<em>L</em><sup>−1</sup>+ AgNO<sub>3</sub> 2 mg·<em>L</em><sup>−1</sup>+ sucrose 3 %, and the induction rate reached 95.83 %. However, different cotyledon cutting methods resulted in different regeneration rates of adventitious buds. Using this regeneration system, the <em>ACS7</em> gene was successfully transferred into luffa by Agrobacterium-mediated transformation.</div></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of an Efficient and Reproducible in Vitro Regeneration and Transformation Protocol for Luffa cylindrica (L.) Roem\",\"authors\":\"Zhengjing Wu, Chenfang Li, Yidian Gao, Hao Zhang, Yuan Long, Bingjie An, Sujuan Liu, Jingjing Wu\",\"doi\":\"10.1016/j.sajb.2024.09.067\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>To establish an efficient in vitro regeneration system and lay a foundation for further research, the effects of shelling, plant hormones, explant position, infection parameters and other factors on the in vitro regeneration of luffa cotyledons were studied. The results showed that the best medium for inducing adventitious buds from luffa cotyledons was Murashige and Skoog (MS) + Thidiazuron (TDZ) 0.3 mg·<em>L</em><sup>−1</sup>+ Indole-3-Butyric acid (IBA) 0.02 mg·<em>L</em><sup>−1</sup>+ AgNO<sub>3</sub> 2 mg·<em>L</em><sup>−1</sup>+ sucrose 3 %, and the induction rate reached 95.83 %. However, different cotyledon cutting methods resulted in different regeneration rates of adventitious buds. Using this regeneration system, the <em>ACS7</em> gene was successfully transferred into luffa by Agrobacterium-mediated transformation.</div></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254629924006227\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254629924006227","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Development of an Efficient and Reproducible in Vitro Regeneration and Transformation Protocol for Luffa cylindrica (L.) Roem
To establish an efficient in vitro regeneration system and lay a foundation for further research, the effects of shelling, plant hormones, explant position, infection parameters and other factors on the in vitro regeneration of luffa cotyledons were studied. The results showed that the best medium for inducing adventitious buds from luffa cotyledons was Murashige and Skoog (MS) + Thidiazuron (TDZ) 0.3 mg·L−1+ Indole-3-Butyric acid (IBA) 0.02 mg·L−1+ AgNO3 2 mg·L−1+ sucrose 3 %, and the induction rate reached 95.83 %. However, different cotyledon cutting methods resulted in different regeneration rates of adventitious buds. Using this regeneration system, the ACS7 gene was successfully transferred into luffa by Agrobacterium-mediated transformation.
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