Shina Sasi , Saranya Krishnan , Preshobha Kodackattumannil , Martin Kottackal , Khaled M.A. Amiri
{"title":"向沙漠豆科植物--红花楹学习,开发抗逆作物","authors":"Shina Sasi , Saranya Krishnan , Preshobha Kodackattumannil , Martin Kottackal , Khaled M.A. Amiri","doi":"10.1016/j.envexpbot.2024.106003","DOIUrl":null,"url":null,"abstract":"<div><div>Plants inhabiting adverse growth conditions compete against stresses by the endogenous regulatory elements <em>viz</em>., promoters and terminators at the ‘right time’ (time-of-stress-act), ‘right place’ (tissue-of-act), and ‘right expression’ (time-of-transcription). Heat stress at the reproductive stage impedes pollen viability and stigma receptiveness, affecting the seed set. <em>Prosopis cineraria,</em> the dominant desert-inhabiting legume tree, is heat- and drought-tolerant. The distribution of heat shock elements in a heat-inducible promoter determines the magnitudes of target gene expression in different tissues/organs. Relative expression of <em>P. cineraria</em> heat shock protein <em>18.2</em> (<em>PcHsp18.2</em>) in alternate months of 2021 displayed the highest expression in summer. The flowers collected in June, the hottest month (47 °C) of 2021, exhibited a high expression of <em>PcHsp18.2.</em> The germination of the pollen collected was 80 %, and the trees eventually set seeds. Comprehensive analysis of the promoter (<em>pPcHSP18.2</em>) and terminator (<em>tPchsp18.2</em>) of <em>PcHsp18.2</em> by expressing the <em>gusA</em> in tobacco exhibited the highest expression under heat stress as similar to the expression of <em>PcHsp18.2</em> in environmental samples. Ectopic expression of <em>gusA</em> under <em>PcHsp18.2</em> promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress. The <em>gusA</em> expression under <em>PcHsp18.2</em> promoter and terminator was high-fold in anther compared to the <em>Lat52</em> and <em>g10</em> (endogenous genes under its promoter and terminator) genes under heat stress. The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing <em>P. cineraria</em> in transgenic plants, enables the development of resilient crops.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106003"},"PeriodicalIF":4.5000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Learning from the desert legume tree, Prosopis cineraria to develop stress-tolerant crops\",\"authors\":\"Shina Sasi , Saranya Krishnan , Preshobha Kodackattumannil , Martin Kottackal , Khaled M.A. Amiri\",\"doi\":\"10.1016/j.envexpbot.2024.106003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Plants inhabiting adverse growth conditions compete against stresses by the endogenous regulatory elements <em>viz</em>., promoters and terminators at the ‘right time’ (time-of-stress-act), ‘right place’ (tissue-of-act), and ‘right expression’ (time-of-transcription). Heat stress at the reproductive stage impedes pollen viability and stigma receptiveness, affecting the seed set. <em>Prosopis cineraria,</em> the dominant desert-inhabiting legume tree, is heat- and drought-tolerant. The distribution of heat shock elements in a heat-inducible promoter determines the magnitudes of target gene expression in different tissues/organs. Relative expression of <em>P. cineraria</em> heat shock protein <em>18.2</em> (<em>PcHsp18.2</em>) in alternate months of 2021 displayed the highest expression in summer. The flowers collected in June, the hottest month (47 °C) of 2021, exhibited a high expression of <em>PcHsp18.2.</em> The germination of the pollen collected was 80 %, and the trees eventually set seeds. Comprehensive analysis of the promoter (<em>pPcHSP18.2</em>) and terminator (<em>tPchsp18.2</em>) of <em>PcHsp18.2</em> by expressing the <em>gusA</em> in tobacco exhibited the highest expression under heat stress as similar to the expression of <em>PcHsp18.2</em> in environmental samples. Ectopic expression of <em>gusA</em> under <em>PcHsp18.2</em> promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress. The <em>gusA</em> expression under <em>PcHsp18.2</em> promoter and terminator was high-fold in anther compared to the <em>Lat52</em> and <em>g10</em> (endogenous genes under its promoter and terminator) genes under heat stress. The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing <em>P. cineraria</em> in transgenic plants, enables the development of resilient crops.</div></div>\",\"PeriodicalId\":11758,\"journal\":{\"name\":\"Environmental and Experimental Botany\",\"volume\":\"228 \",\"pages\":\"Article 106003\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental and Experimental Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0098847224003617\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental and Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0098847224003617","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Learning from the desert legume tree, Prosopis cineraria to develop stress-tolerant crops
Plants inhabiting adverse growth conditions compete against stresses by the endogenous regulatory elements viz., promoters and terminators at the ‘right time’ (time-of-stress-act), ‘right place’ (tissue-of-act), and ‘right expression’ (time-of-transcription). Heat stress at the reproductive stage impedes pollen viability and stigma receptiveness, affecting the seed set. Prosopis cineraria, the dominant desert-inhabiting legume tree, is heat- and drought-tolerant. The distribution of heat shock elements in a heat-inducible promoter determines the magnitudes of target gene expression in different tissues/organs. Relative expression of P. cineraria heat shock protein 18.2 (PcHsp18.2) in alternate months of 2021 displayed the highest expression in summer. The flowers collected in June, the hottest month (47 °C) of 2021, exhibited a high expression of PcHsp18.2. The germination of the pollen collected was 80 %, and the trees eventually set seeds. Comprehensive analysis of the promoter (pPcHSP18.2) and terminator (tPchsp18.2) of PcHsp18.2 by expressing the gusA in tobacco exhibited the highest expression under heat stress as similar to the expression of PcHsp18.2 in environmental samples. Ectopic expression of gusA under PcHsp18.2 promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress. The gusA expression under PcHsp18.2 promoter and terminator was high-fold in anther compared to the Lat52 and g10 (endogenous genes under its promoter and terminator) genes under heat stress. The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing P. cineraria in transgenic plants, enables the development of resilient crops.
期刊介绍:
Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment.
In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief.
The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB.
The areas covered by the Journal include:
(1) Responses of plants to heavy metals and pollutants
(2) Plant/water interactions (salinity, drought, flooding)
(3) Responses of plants to radiations ranging from UV-B to infrared
(4) Plant/atmosphere relations (ozone, CO2 , temperature)
(5) Global change impacts on plant ecophysiology
(6) Biotic interactions involving environmental factors.