基于单细胞拉曼光谱技术评估面包酵母的生理特性

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xi Sun , Xin Zhou , Ran Yu , Xiaofang Zhou , Jun Zhang , Teng Xu , Jianmei Wang , Mengqi Li , Xiaoting Li , Min Zhang , Jian Xu , Jia Zhang
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引用次数: 0

摘要

随着酵母发酵行业的快速发展,需要一种集效率、准确性、灵敏度和成本效益于一体的综合性商业酵母质量评估方法。本研究开发了一种基于单细胞拉曼技术的新型酵母质量评估方法,并与传统方法进行了对比。研究结果表明,这两种方法在测量生理指标(包括细胞活力和细胞内三卤素含量)方面有明显的相关性(皮尔逊相关系数平均为 0.933),证明了拉曼方法与传统方法相比的可信度。此外,在测量酵母细胞活力时,拉曼法对有活力但无法培养的细胞的灵敏度更高(灵敏度高出 17.9%)。根据对酵母细胞代谢活性水平(MAL)的精确定量分析,细胞活力可在群体和单细胞水平上精确定量,从而更全面地评估酵母发酵性能。总之,单细胞拉曼方法集酵母质量评估的可信度、可行性、准确性和灵敏度于一体,为活细胞酵母产品的质量评估提供了新的技术框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology
With rapid progress in the yeast fermentation industry, a comprehensive commercial yeast quality assessment approach integrating efficiency, accuracy, sensitivity, and cost-effectiveness is required. In this study, a new yeast quality assessment method based on single-cell Raman technology was developed and contrasted with traditional methods. The findings demonstrated significant associations (Pearson correlation coefficient of 0.933 on average) between the two methods in measuring physiological indicators, including cell viability and intracellular trehalose content, demonstrating the credibility of the Raman method compared to the traditional method. Furthermore, the sensitivity of the Raman method in viable but non-culturable cells was higher in measuring yeast cell viability (17.9 % more sensitive). According to the accurate quantitative analysis of metabolic activity level (MAL) of yeast cells, the cell vitality was accurately quantified at population and single-cell levels, offering a more comprehensive assessment of yeast fermentation performance. Overall, the single-cell Raman method integrates credibility, feasibility, accuracy, and sensitivity in yeast quality assessment, offering a new technological framework for quality assessments of live-cell yeast products.
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来源期刊
Synthetic and Systems Biotechnology
Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
6.90
自引率
12.50%
发文量
90
审稿时长
67 days
期刊介绍: Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.
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