{"title":"利用空间隔离的金铈进行级联酶模拟,实现超氧阴离子的双模式检测","authors":"","doi":"10.1016/j.bios.2024.116847","DOIUrl":null,"url":null,"abstract":"<div><div>Metal-semiconductor nanozyme of dumbbell Au-CeO<sub>2</sub> with spatially separated heterostructure has cascade superoxide dismutase (SOD)-like and peroxidase (POD)-like activities for superoxide anions detection. It was synthesized by selective growth of CeO<sub>2</sub> at the ends of Au nanorod (Au NR). Taking advantage of the excellent local surface plasmon resonance (LSPR) effect of Au NR, the spatially separated Au-CeO<sub>2</sub> has a higher photothermal effect than the continuously growing core-shell structure of Au@CeO<sub>2</sub>. Meanwhile, the hot electrons of Au NR could transfer to CeO<sub>2</sub> under 808 nm laser irradiation, changing the ratio of Ce<sup>3+</sup>/Ce<sup>4+</sup> redox couples over CeO<sub>2</sub> and facilitating H<sub>2</sub>O<sub>2</sub> decomposition thus enhancing POD-like activity. Based on the SOD-like activity of Au-CeO<sub>2</sub>, superoxide anion (O<sub>2</sub><sup>·−</sup>) can be transformed into hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Dual-mode including absorbance and temperature sensing detection of O<sub>2</sub><sup>·−</sup>, with the detection range from nM to μM i.e., 0.1–150 μM and LOD of 0.033 μM (S/N = 3) was achieved through the cascade catalysis and photothermal effect. The as-proposed method was applicable to both cancer and normal cell samples with satisfactory accuracy and recovery.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7000,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cascade enzyme-mimicking with spatially separated gold-ceria for dual-mode detection of superoxide anions\",\"authors\":\"\",\"doi\":\"10.1016/j.bios.2024.116847\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Metal-semiconductor nanozyme of dumbbell Au-CeO<sub>2</sub> with spatially separated heterostructure has cascade superoxide dismutase (SOD)-like and peroxidase (POD)-like activities for superoxide anions detection. It was synthesized by selective growth of CeO<sub>2</sub> at the ends of Au nanorod (Au NR). Taking advantage of the excellent local surface plasmon resonance (LSPR) effect of Au NR, the spatially separated Au-CeO<sub>2</sub> has a higher photothermal effect than the continuously growing core-shell structure of Au@CeO<sub>2</sub>. Meanwhile, the hot electrons of Au NR could transfer to CeO<sub>2</sub> under 808 nm laser irradiation, changing the ratio of Ce<sup>3+</sup>/Ce<sup>4+</sup> redox couples over CeO<sub>2</sub> and facilitating H<sub>2</sub>O<sub>2</sub> decomposition thus enhancing POD-like activity. Based on the SOD-like activity of Au-CeO<sub>2</sub>, superoxide anion (O<sub>2</sub><sup>·−</sup>) can be transformed into hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Dual-mode including absorbance and temperature sensing detection of O<sub>2</sub><sup>·−</sup>, with the detection range from nM to μM i.e., 0.1–150 μM and LOD of 0.033 μM (S/N = 3) was achieved through the cascade catalysis and photothermal effect. The as-proposed method was applicable to both cancer and normal cell samples with satisfactory accuracy and recovery.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2024-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566324008546\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566324008546","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
Cascade enzyme-mimicking with spatially separated gold-ceria for dual-mode detection of superoxide anions
Metal-semiconductor nanozyme of dumbbell Au-CeO2 with spatially separated heterostructure has cascade superoxide dismutase (SOD)-like and peroxidase (POD)-like activities for superoxide anions detection. It was synthesized by selective growth of CeO2 at the ends of Au nanorod (Au NR). Taking advantage of the excellent local surface plasmon resonance (LSPR) effect of Au NR, the spatially separated Au-CeO2 has a higher photothermal effect than the continuously growing core-shell structure of Au@CeO2. Meanwhile, the hot electrons of Au NR could transfer to CeO2 under 808 nm laser irradiation, changing the ratio of Ce3+/Ce4+ redox couples over CeO2 and facilitating H2O2 decomposition thus enhancing POD-like activity. Based on the SOD-like activity of Au-CeO2, superoxide anion (O2·−) can be transformed into hydrogen peroxide (H2O2). Dual-mode including absorbance and temperature sensing detection of O2·−, with the detection range from nM to μM i.e., 0.1–150 μM and LOD of 0.033 μM (S/N = 3) was achieved through the cascade catalysis and photothermal effect. The as-proposed method was applicable to both cancer and normal cell samples with satisfactory accuracy and recovery.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.