调控免疫细胞和非免疫细胞的 CRISPR-Cas9 传递策略

IF 79.8 1区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nature Reviews Materials Pub Date : 2024-10-16 DOI:10.1038/s41578-024-00725-7

Shahad K. Alsaiari, Behnaz Eshaghi, Bujie Du, Maria Kanelli, Gary Li, Xunhui Wu, Linzixuan Zhang, Mehr Chaddah, Alicia Lau, Xin Yang, Robert Langer, Ana Jaklenec

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引用次数: 0

摘要

CRISPR-Cas9 基因组编辑技术是对免疫细胞进行基因工程和调节免疫系统的一种前景广阔的工具。虽然免疫细胞的体外基因组编辑已进入临床试验阶段，但体内应用仍受到 CRISPR-Cas9 成分不稳定和通过循环向免疫细胞传递效率低的限制。在本综述中，我们总结了向非免疫细胞和免疫细胞输送 CRISPR-Cas9 成分的体内外策略。我们回顾了在非免疫细胞方面取得的进展，因为这些进展提供了可用于推进免疫细胞研究的启示。我们还讨论了使用 CRISPR-Cas9 基因组编辑技术调节免疫系统的原理和挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

CRISPR–Cas9 delivery strategies for the modulation of immune and non-immune cells

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CRISPR–Cas9 delivery strategies for the modulation of immune and non-immune cells

CRISPR–Cas9 genome editing technology is a promising tool for genetically engineering immune cells and modulating immune systems. Although ex vivo genome editing of immune cells has reached clinical trials, in vivo application is still restricted by the instability and inefficient delivery of CRISPR–Cas9 components to immune cells through circulation. In this Review, we summarize ex vivo and in vivo strategies to deliver CRISPR–Cas9 components to both non-immune and immune cells. We review the progress made in non-immune cells because it offers insights that can be applied to advancing research in immune cells. We also discuss principles and challenges of immune system modulation using CRISPR–Cas9 genome editing technology.

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来源期刊

Nature Reviews Materials Materials Science-Biomaterials

CiteScore

119.40

自引率

0.40%

发文量

107

期刊介绍： Nature Reviews Materials is an online-only journal that is published weekly. It covers a wide range of scientific disciplines within materials science. The journal includes Reviews, Perspectives, and Comments. Nature Reviews Materials focuses on various aspects of materials science, including the making, measuring, modelling, and manufacturing of materials. It examines the entire process of materials science, from laboratory discovery to the development of functional devices.