微生物群对体外共培养模型中艰难梭菌的生长和基因表达的影响

IF 3.9 3区 生物学 Q2 MICROBIOLOGY
Elisa Martinez, Noémie Berg, Cristina Rodriguez, Georges Daube, Bernard Taminiau
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引用次数: 0

摘要

艰难梭菌是一种厌氧、孢子形成型革兰氏阳性致病菌。本研究旨在利用体外共培养模型分析两种健康粪便微生物群样本对艰难梭菌基因表达和生长的影响。内室与艰难梭菌聚合酶链反应(PCR)核型 078 的孢子共培养,外室则含有供体的粪便样本,以模拟微生物群(FD1 和 FD2)。不含粪便的平板作为对照(CT)。对内仓样本进行了 RNA-Seq 和定量 PCR 确认。在有微生物群存在的情况下,观察到了基因表达的相似性。12 小时后,在微生物群存在的情况下,与发芽、孢子生成、毒素产生和生长相关的基因表达下调。24 小时后,在缺铁环境中,艰难梭菌激活了多个基因以抵消缺铁。在有供体 1(FD1)微生物群存在的情况下,与 12 小时相比,24 小时内与发芽和孢子形成相关的基因表达上调。这项研究证实了之前的发现,即艰难梭菌可以利用乙醇胺作为主要营养源。为了进一步研究这种相互作用,未来的研究将使用简化的人工细菌联合体共培养模型,而不是粪便样本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Influence of microbiota on the growth and gene expression of Clostridioides difficile in an in vitro coculture model

Influence of microbiota on the growth and gene expression of Clostridioides difficile in an in vitro coculture model

Clostridioides difficile is an anaerobic, spore-forming, Gram-positive pathogenic bacterium. This study aimed to analyze the effect of two samples of healthy fecal microbiota on C. difficile gene expression and growth using an in vitro coculture model. The inner compartment was cocultured with spores of the C. difficile polymerase chain reaction (PCR)-ribotype 078, while the outer compartment contained fecal samples from donors to mimic the microbiota (FD1 and FD2). A fecal-free plate served as a control (CT). RNA-Seq and quantitative PCR confirmation were performed on the inner compartment sample. Similarities in gene expression were observed in the presence of the microbiota. After 12 h, the expression of genes associated with germination, sporulation, toxin production, and growth was downregulated in the presence of the microbiota. At 24 h, in an iron-deficient environment, C. difficile activated several genes to counteract iron deficiency. The expression of genes associated with germination and sporulation was upregulated at 24 h compared with 12 h in the presence of microbiota from donor 1 (FD1). This study confirmed previous findings that C. difficile can use ethanolamine as a primary nutrient source. To further investigate this interaction, future studies will use a simplified coculture model with an artificial bacterial consortium instead of fecal samples.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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