从波兰桦树粘液流中分离出的新 Phaffia rhodozyma 酵母菌菌株的特征

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Anna M. Kot, Katarzyna Pobiega, Marek Kieliszek, Katarzyna Michalak, Stanisław Błażejak
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引用次数: 0

摘要

最近在波兰分离出了三株新的 Phaffia rhodozyma 酵母菌。本研究的目的是对这些菌株进行表型鉴定,并将它们与参考菌株的特性进行比较。此外,还测定了这些菌株类胡萝卜素生物合成的潜力,这取决于培养基中的温度、碳源和氮源。还通过 MALDI-TOF MS 对 Phaffia rhodozyma 酵母菌进行了鉴定。各菌株的细胞形态略有不同。所有菌株都通过出芽进行无性繁殖,并形成球形衣壳孢子。没有观察到有性生殖能力。生理测试表明,参考菌株和分离菌株之间存在微小差异,这可能是由于它们最初分离出来的栖息地具有地理特异性。蛋白质光谱分析显示,受测酵母分离物有七个强度不同的共同峰,质量分别为 2200、2369、3213、3628、3776、3921 和 4710 m/z。此外,还发现了其他依赖于菌株的光谱。类胡萝卜素的合成量随使用的碳源、氮源和温度而变化。类胡萝卜素产量最高的是 P. rhodozyma CMIFS 102 分离物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characteristic of new Phaffia rhodozyma yeast strains isolated from birch slime fluxes in Poland

Three new strains of Phaffia rhodozyma yeast have recently been isolated in Poland. The aim of this study was to phenotypically characterize these strains and to compare them with the properties of the reference strain. The potential for carotenoid biosynthesis in these strains was also determined, depending on temperature, carbon, and nitrogen sources in the medium. Phaffia rhodozyma yeasts were also identified by MALDI-TOF MS. There were minor differences in cell morphology among the strains. All strains reproduced asexually by budding and formed spherical chlamydospores. No ability for sexual reproduction was observed. Physiological tests showed minor variations between the reference strain and the isolates, likely due to the geographical specificity of the habitat from which they were originally isolated. Analysis of protein spectra showed that the tested yeast isolates had seven common peaks of different intensities, with masses at 2200, 2369, 3213, 3628, 3776, 3921, and 4710 m/z. Moreover, additional strain-dependent spectra were found. The amount of synthesized carotenoids varied with the carbon and nitrogen sources used, as well as the temperature. The best producer of carotenoids was the P. rhodozyma CMIFS 102 isolate.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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