揭示精氨酸代谢在生理学和病理学中的功能多样性

IF 27.7 1区 生物学 Q1 CELL BIOLOGY
Rui Li, Yan Li, Kun Jiang, Lijuan Zhang, Ting Li, Aihua Zhao, Zhuo Zhang, Yale Xia, Kun Ge, Yaqiong Chen, Chengnuo Wang, Weitao Tang, Shuning Liu, Xiaoxi Lin, Yuqin Song, Jie Mei, Chun Xiao, Aoxue Wang, Yejun Zou, Xie Li, Yuzheng Zhao
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引用次数: 0

摘要

精氨酸是代谢能力最强的氨基酸之一,在多种生物和病理过程中发挥着关键作用;然而,原位灵敏跟踪精氨酸动态在技术上仍具有挑战性。在这里,我们设计了高性能的荧光生物传感器(对精氨酸敏感(STAR))来阐明细胞、小鼠和临床样本中的精氨酸代谢。利用 STAR,我们展示了不同氨基酸在调节细胞内和细胞外精氨酸水平方面的作用。STAR 能够活细胞监测巨噬细胞活化、吞噬、排泄和衰老过程中的精氨酸波动,并揭示了细胞衰老取决于精氨酸的可用性。此外,一种基于 STAR 的简单快速的检测方法显示,血清精氨酸水平随着年龄的增长呈上升趋势,而血清精氨酸水平的升高是判别白癜风进展和严重程度的一个潜在指标。总之,我们的研究为精氨酸的代谢和功能作用及其在诊断和治疗中的应用潜力提供了重要的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology
Arginine is one of the most metabolically versatile amino acids and plays pivotal roles in diverse biological and pathological processes; however, sensitive tracking of arginine dynamics in situ remains technically challenging. Here, we engineer high-performance fluorescent biosensors, denoted sensitive to arginine (STAR), to illuminate arginine metabolism in cells, mice, and clinical samples. Utilizing STAR, we demonstrate the effects of different amino acids in regulating intra- and extracellular arginine levels. STAR enabled live-cell monitoring of arginine fluctuations during macrophage activation, phagocytosis, efferocytosis, and senescence and revealed cellular senescence depending on arginine availability. Moreover, a simple and fast assay based on STAR revealed that serum arginine levels tended to increase with age, and the elevated serum arginine level is a potential indicator for discriminating the progression and severity of vitiligo. Collectively, our study provides important insights into the metabolic and functional roles of arginine, as well as its potential in diagnostic and therapeutic applications.
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来源期刊
Cell metabolism
Cell metabolism 生物-内分泌学与代谢
CiteScore
48.60
自引率
1.40%
发文量
173
审稿时长
2.5 months
期刊介绍: Cell Metabolism is a top research journal established in 2005 that focuses on publishing original and impactful papers in the field of metabolic research.It covers a wide range of topics including diabetes, obesity, cardiovascular biology, aging and stress responses, circadian biology, and many others. Cell Metabolism aims to contribute to the advancement of metabolic research by providing a platform for the publication and dissemination of high-quality research and thought-provoking articles.
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