Timothy Byaruhanga , Stuart Astbury , Jack D. Hill , Theocharis Tsoleridis , Joseph G. Chappell , John T. Kayiwa , Irene J. Ataliba , Annet M. Nankya , Jonathan K. Ball , Julius J. Lutwama , C. Patrick McClure
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Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018
We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.
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