利用极性分区二维液相色谱-质谱法全面鉴定丹参中的活性成分

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2024-10-06 DOI:10.1016/j.chroma.2024.465424

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引用次数: 0

摘要

丹参是一种广泛使用的传统中药，含有疏水性二萜和亲水性酚酸的复杂基质，给综合分析带来了巨大挑战。本研究建立了一种在线极性分区二维液相色谱-质谱联用（2D-LC-MS）方法，用于丹参中亲脂性和亲水性活性成分的综合分析。该方法整合了亲水作用液相色谱法（HILIC）和反相液相色谱法（RPLC），有助于高效分离各种极性的化合物。该方法采用了在线稀释策略，最大程度地减少了样品损失，提高了该方法在复杂草药基质的质量控制和化学表征方面的实用性。与其他液相色谱法相比，该方法显著提高了分析物的覆盖率、分辨率和分析效率。在最佳条件下，成功鉴定了 150 种活性成分，包括丹参中新发现的 33 种化合物。此外，该在线方法还被用于定量检测不同来源丹参的 16 个质量标记物。结果表明，该在线方法具有替代现有技术的潜力，在传统中药研究中具有更广泛的适用性。

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Comprehensive characterization of active components in Salvia miltiorrhiza using polarity-partitioned two-dimensional liquid chromatography coupled with mass spectrometry

Salvia miltiorrhiza, a widely used traditional Chinese medicine, contains a complex matrix of hydrophobic diterpenoids and hydrophilic phenolic acids, presenting significant challenges in comprehensive analysis. In this study, an online polarity-partitioned two-dimensional liquid chromatography coupled with mass spectrometry (2D-LC-MS) method was developed for comprehensive analysis of both lipophilic and hydrophilic active components in Salvia miltiorrhiza. The method integrated hydrophilic interaction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC), facilitating the efficient separation of compounds across a wide range of polarities. An online dilution strategy was implemented, minimizing sample loss and enhancing the method's utility for quality control and chemical characterization of complex herbal matrices. Compared with other LC methods, this approach significantly improved analyte coverage, resolution, and analysis efficiency. Under optimal conditions, 150 active components were successfully identified, including 33 compounds newly discovered in Salvia miltiorrhiza. Additionally, the validated online method was applied to the quantitative determination of 16 quality markers of Salvia miltiorrhiza from different sources. The results demonstrated the online method's potential as a superior alternative to existing techniques, offering broader applicability in traditional Chinese medicine research.

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.