Jiaojiao Liu , Jiayue Liu , Shuwen Wang , Qiang Fu , Taogetao Baoyin , Zhihua Bao , Frank Yonghong Li
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Our results indicated that mowing significantly increased the BNF rate (<em>P</em> < 0.01) from 11.48 g N ha<sup>−1</sup> d<sup>−1</sup> (NM) to 25.16 g N ha<sup>−1</sup> d<sup>−1</sup> (MO); mowing also significantly increased average N fixation activity per <em>nifH</em> gene (<em>P</em> < 0.05), while reduced the <em>nifH</em> gene abundance (<em>P</em> < 0.05). The <em>nifH</em> gene abundance was not significantly correlated with the BNF rate (<em>P</em> > 0.05), suggesting that the <em>nifH</em> gene abundance based on DNA analysis was not indicative for BNF rate; while soil ammonium nitrogen (<span><math><msubsup><mi>NH</mi><mn>4</mn><mo>+</mo></msubsup></math></span>-N) content was identified by stepwise multiple regression the only variable that can significantly explain the variation in BNF rate. Our results suggest that soil <span><math><msubsup><mi>NH</mi><mn>4</mn><mo>+</mo></msubsup></math></span>-N content is the most efficient predictor of BNF rate instead of <em>nifH</em> gene abundance, and it is more crucial to quantify the impacts of soil <span><math><msubsup><mi>NH</mi><mn>4</mn><mo>+</mo></msubsup></math></span>-N than the effects of diazotrophic abundance in predicting the changes in BNF rate in response to mowing management in semiarid grassland.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"203 ","pages":"Article 105690"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Soil biological nitrogen fixation is closely associated with soil ammonium nitrogen content in a mowing semiarid natural grassland\",\"authors\":\"Jiaojiao Liu , Jiayue Liu , Shuwen Wang , Qiang Fu , Taogetao Baoyin , Zhihua Bao , Frank Yonghong Li\",\"doi\":\"10.1016/j.apsoil.2024.105690\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Soil biological nitrogen fixation (BNF) plays a significant role in N input in terrestrial ecosystems, and can be largely altered by management. In semi-arid region of Northern China, natural grasslands rely on soil BNF to maintain nitrogen input under long-mowing, but the effects of mowing on BNF remain unclear. Here we assessed the soil BNF rate and the <em>nifH</em> gene abundance in soil (0–10 cm) of the grasslands subject to long-term (19 years) annual mowing (MO) versus no mowing (NM) in a semiarid natural steppe grassland. Our results indicated that mowing significantly increased the BNF rate (<em>P</em> < 0.01) from 11.48 g N ha<sup>−1</sup> d<sup>−1</sup> (NM) to 25.16 g N ha<sup>−1</sup> d<sup>−1</sup> (MO); mowing also significantly increased average N fixation activity per <em>nifH</em> gene (<em>P</em> < 0.05), while reduced the <em>nifH</em> gene abundance (<em>P</em> < 0.05). The <em>nifH</em> gene abundance was not significantly correlated with the BNF rate (<em>P</em> > 0.05), suggesting that the <em>nifH</em> gene abundance based on DNA analysis was not indicative for BNF rate; while soil ammonium nitrogen (<span><math><msubsup><mi>NH</mi><mn>4</mn><mo>+</mo></msubsup></math></span>-N) content was identified by stepwise multiple regression the only variable that can significantly explain the variation in BNF rate. 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引用次数: 0
摘要
土壤生物固氮(BNF)在陆地生态系统的氮输入中发挥着重要作用,并且在很大程度上会因管理而改变。在华北半干旱地区,天然草地在长期刈割的情况下依靠土壤生物固氮作用维持氮素输入,但刈割对土壤生物固氮作用的影响尚不清楚。在此,我们评估了在半干旱天然草原上长期(19年)每年刈割(MO)与不刈割(NM)草地土壤(0-10厘米)中的土壤BNF率和nifH基因丰度。我们的结果表明,刈割显著提高了BNF率(P < 0.01),从11.48 g N ha-1 d-1 (NM)提高到25.16 g N ha-1 d-1 (MO);刈割还显著提高了每个nifH基因的平均固氮活性(P < 0.05),同时降低了nifH基因丰度(P < 0.05)。nifH 基因丰度与 BNF 速率无显著相关性(P >;0.05),表明基于 DNA 分析的 nifH 基因丰度对 BNF 速率无指示作用;而土壤铵态氮(NH4+-N)含量经逐步多元回归被确定为唯一能显著解释 BNF 速率变化的变量。我们的研究结果表明,土壤 NH4+-N 含量而非 nifH 基因丰度是预测 BNF 速率的最有效指标,在预测半干旱草地刈割管理对 BNF 速率的影响时,量化土壤 NH4+-N 的影响比重氮营养体丰度的影响更为重要。
Soil biological nitrogen fixation is closely associated with soil ammonium nitrogen content in a mowing semiarid natural grassland
Soil biological nitrogen fixation (BNF) plays a significant role in N input in terrestrial ecosystems, and can be largely altered by management. In semi-arid region of Northern China, natural grasslands rely on soil BNF to maintain nitrogen input under long-mowing, but the effects of mowing on BNF remain unclear. Here we assessed the soil BNF rate and the nifH gene abundance in soil (0–10 cm) of the grasslands subject to long-term (19 years) annual mowing (MO) versus no mowing (NM) in a semiarid natural steppe grassland. Our results indicated that mowing significantly increased the BNF rate (P < 0.01) from 11.48 g N ha−1 d−1 (NM) to 25.16 g N ha−1 d−1 (MO); mowing also significantly increased average N fixation activity per nifH gene (P < 0.05), while reduced the nifH gene abundance (P < 0.05). The nifH gene abundance was not significantly correlated with the BNF rate (P > 0.05), suggesting that the nifH gene abundance based on DNA analysis was not indicative for BNF rate; while soil ammonium nitrogen (-N) content was identified by stepwise multiple regression the only variable that can significantly explain the variation in BNF rate. Our results suggest that soil -N content is the most efficient predictor of BNF rate instead of nifH gene abundance, and it is more crucial to quantify the impacts of soil -N than the effects of diazotrophic abundance in predicting the changes in BNF rate in response to mowing management in semiarid grassland.
期刊介绍:
Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.