毛细管电泳和微尺度液相色谱中的荧光检测装置：过去十年的发展

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2024-10-08 DOI:10.1016/j.trac.2024.118001

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引用次数: 0

摘要

经过适当调整和操作的荧光检测器可提供比质谱仪更高的灵敏度，其灵敏度和选择性也远远优于吸光检测器。因此，各种荧光检测器正被不断开发和改造，用于高效液相色谱和毛细管电泳。本微型综述总结了适用于毛细管分离系统的荧光检测器的最新发展，重点介绍了光学排列、激发光源、检测区外观、多波长检测方案以及与其他检测原理相结合的荧光监测等技术细节。不包括微芯片配置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fluorescence detection setups in capillary electrophoresis and microscale liquid chromatography: Developments over the past decade

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Fluorescence detection setups in capillary electrophoresis and microscale liquid chromatography: Developments over the past decade

Properly adjusted and operated fluorescence detectors may provide higher sensitivity than mass spectrometers and far better sensitivity and selectivity than absorbance detectors. Therefore, various fluorescence detectors are being continuously developed and adapted for high-performance liquid chromatography and capillary electrophoresis. This mini-review summarizes recent developments in fluorescence detectors applicable in capillary scale separation systems with emphasis on the technical details of the optical arrangements, excitation light sources, appearance of the detection zone, multi-wavelength detection schemes, and fluorescence monitoring in tandem with other detection principles. Microchip configurations are not covered.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.