通过环介导等温反应检测新生儿血液样本中基于 wabG 基因的肺炎克雷伯菌的诊断准确性评估

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-05 DOI:10.1016/j.diagmicrobio.2024.116552

Shukla Banerjee , Mithun H. K , K Sudhakara Prasad , Rajesh P Shastry

{"title":"通过环介导等温反应检测新生儿血液样本中基于 wabG 基因的肺炎克雷伯菌的诊断准确性评估","authors":"Shukla Banerjee , Mithun H. K , K Sudhakara Prasad , Rajesh P Shastry","doi":"10.1016/j.diagmicrobio.2024.116552","DOIUrl":null,"url":null,"abstract":"<div><div>Neonatal sepsis is a significant problem in developing nations, but current gold-standard diagnostic methods, such as blood culture, is slow and time-consuming. Here, we describe the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay that targets the <em>wabG</em> gene in the lipopolysaccharide region of <em>K. pneumoniae</em>, offering a limit of detection (LOD) of 40 CFU/ml with specificity for <em>K. pneumoniae</em> compared to other non-<em>Klebsiella</em> strains. The sensitivity and specificity of the LAMP assay were found to be 90 % and 100 %, respectively, with a positive predictive value of 100 % and a negative predictive value of 96.47 %. The LAMP assay demonstrated a significantly shorter turnaround time of 1 h. The LAMP assay was found to be simpler, quicker, and more sensitive than traditional detection techniques such as PCR and blood culture.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116552"},"PeriodicalIF":2.1000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of diagnostic accuracy of the wabG gene based Klebsiella pneumoniae detection by loop-mediated isothermal reaction in neonatal blood sample\",\"authors\":\"Shukla Banerjee , Mithun H. K , K Sudhakara Prasad , Rajesh P Shastry\",\"doi\":\"10.1016/j.diagmicrobio.2024.116552\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Neonatal sepsis is a significant problem in developing nations, but current gold-standard diagnostic methods, such as blood culture, is slow and time-consuming. Here, we describe the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay that targets the <em>wabG</em> gene in the lipopolysaccharide region of <em>K. pneumoniae</em>, offering a limit of detection (LOD) of 40 CFU/ml with specificity for <em>K. pneumoniae</em> compared to other non-<em>Klebsiella</em> strains. The sensitivity and specificity of the LAMP assay were found to be 90 % and 100 %, respectively, with a positive predictive value of 100 % and a negative predictive value of 96.47 %. The LAMP assay demonstrated a significantly shorter turnaround time of 1 h. The LAMP assay was found to be simpler, quicker, and more sensitive than traditional detection techniques such as PCR and blood culture.</div></div>\",\"PeriodicalId\":11329,\"journal\":{\"name\":\"Diagnostic microbiology and infectious disease\",\"volume\":\"110 4\",\"pages\":\"Article 116552\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic microbiology and infectious disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0732889324003778\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889324003778","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

新生儿败血症是发展中国家的一个重大问题，但目前的金标准诊断方法（如血液培养）既缓慢又耗时。在此，我们介绍了针对肺炎克雷伯菌脂多糖区 wabG 基因开发的比色环介导等温扩增（LAMP）检测方法，与其他非克雷伯菌株相比，该方法对肺炎克雷伯菌的检测限（LOD）为 40 CFU/ml，且具有特异性。LAMP检测法的灵敏度和特异性分别为90%和100%，阳性预测值为100%，阴性预测值为96.47%。与 PCR 和血液培养等传统检测技术相比，LAMP 检测更简单、更快速、更灵敏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Evaluation of diagnostic accuracy of the wabG gene based Klebsiella pneumoniae detection by loop-mediated isothermal reaction in neonatal blood sample

Neonatal sepsis is a significant problem in developing nations, but current gold-standard diagnostic methods, such as blood culture, is slow and time-consuming. Here, we describe the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay that targets the wabG gene in the lipopolysaccharide region of K. pneumoniae, offering a limit of detection (LOD) of 40 CFU/ml with specificity for K. pneumoniae compared to other non-Klebsiella strains. The sensitivity and specificity of the LAMP assay were found to be 90 % and 100 %, respectively, with a positive predictive value of 100 % and a negative predictive value of 96.47 %. The LAMP assay demonstrated a significantly shorter turnaround time of 1 h. The LAMP assay was found to be simpler, quicker, and more sensitive than traditional detection techniques such as PCR and blood culture.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.