编码生长因子的基因多态性与正畸患者牙髓钙化的研究

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2024-10-02 DOI:10.1016/j.jobcr.2024.09.013

Sandra Regina Santos Meyfarth , Iago Ramirez , Alice Corrêa Silva-Sousa , Peter Proff , Marilisa Carneiro Leão Gabardo , Manoel Damião Sousa-Neto , Flares Baratto-Filho , Erika Calvano Küchler , Leonardo Santos Antunes , Christian Kirschneck

{"title":"编码生长因子的基因多态性与正畸患者牙髓钙化的研究","authors":"Sandra Regina Santos Meyfarth , Iago Ramirez , Alice Corrêa Silva-Sousa , Peter Proff , Marilisa Carneiro Leão Gabardo , Manoel Damião Sousa-Neto , Flares Baratto-Filho , Erika Calvano Küchler , Leonardo Santos Antunes , Christian Kirschneck","doi":"10.1016/j.jobcr.2024.09.013","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and objectives</h3><div>Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in <em>epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), transforming growth factor-beta 1 (TGFβ1), and transforming growth factor-beta receptor 2 (TGFβR2)</em> are associated with a risk of dental pulp calcifications in orthodontic patients.</div></div><div><h3>Materials and methods</h3><div>Digital orthopantomography (OPG) and genomic DNA from 132 patients were analyzed in this cross-sectional study. Pulp calcification was observed in the maxillary and mandibular first molars. Genomic DNA extracted from saliva cells was used to genotype eight genetic polymorphisms using real-time polymerase chain reaction: <em>EGF</em> (rs2237051 and rs4444903), <em>EGFR</em> (rs2227983 and rs763317), <em>TGFβ1</em> (rs1800469 and rs4803455), and <em>TGFβR2</em> (rs3087465 and rs764522). The association between pulp calcification and genetic polymorphisms was analyzed using allelic and genotypic distributions, and haplotype frequencies (<em>P</em> < 0.05).</div></div><div><h3>Results</h3><div>The prevalence of pulp calcification was 42.4 % in 490 molars. Genotypic analysis and allelic distribution showed no statistically significant association between the evaluated growth factors and molar calcification (<em>P</em> > 0.05). No haplotype combinations showed a statistically significant difference (<em>P</em> > 0.05).</div></div><div><h3>Conclusion</h3><div>The genetic polymorphisms investigated were not associated with dental pulp calcification in orthodontic patients. Further studies should investigate other polymorphisms in genes encoding growth factors.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"14 6","pages":"Pages 712-719"},"PeriodicalIF":0.0000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients\",\"authors\":\"Sandra Regina Santos Meyfarth , Iago Ramirez , Alice Corrêa Silva-Sousa , Peter Proff , Marilisa Carneiro Leão Gabardo , Manoel Damião Sousa-Neto , Flares Baratto-Filho , Erika Calvano Küchler , Leonardo Santos Antunes , Christian Kirschneck\",\"doi\":\"10.1016/j.jobcr.2024.09.013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and objectives</h3><div>Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in <em>epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), transforming growth factor-beta 1 (TGFβ1), and transforming growth factor-beta receptor 2 (TGFβR2)</em> are associated with a risk of dental pulp calcifications in orthodontic patients.</div></div><div><h3>Materials and methods</h3><div>Digital orthopantomography (OPG) and genomic DNA from 132 patients were analyzed in this cross-sectional study. Pulp calcification was observed in the maxillary and mandibular first molars. Genomic DNA extracted from saliva cells was used to genotype eight genetic polymorphisms using real-time polymerase chain reaction: <em>EGF</em> (rs2237051 and rs4444903), <em>EGFR</em> (rs2227983 and rs763317), <em>TGFβ1</em> (rs1800469 and rs4803455), and <em>TGFβR2</em> (rs3087465 and rs764522). The association between pulp calcification and genetic polymorphisms was analyzed using allelic and genotypic distributions, and haplotype frequencies (<em>P</em> < 0.05).</div></div><div><h3>Results</h3><div>The prevalence of pulp calcification was 42.4 % in 490 molars. Genotypic analysis and allelic distribution showed no statistically significant association between the evaluated growth factors and molar calcification (<em>P</em> > 0.05). No haplotype combinations showed a statistically significant difference (<em>P</em> > 0.05).</div></div><div><h3>Conclusion</h3><div>The genetic polymorphisms investigated were not associated with dental pulp calcification in orthodontic patients. Further studies should investigate other polymorphisms in genes encoding growth factors.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"14 6\",\"pages\":\"Pages 712-719\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S221242682400143X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221242682400143X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

背景和目的牙槽骨钙化与许多因素和诱发因素有关，包括个体遗传倾向和正畸力。本研究旨在探讨表皮生长因子（EGF）、表皮生长因子受体（EGFR1）、转化生长因子-β1（TGFβ1）和转化生长因子-β受体 2（TGFβR2）的遗传多态性是否与正畸患者牙髓钙化的风险有关。在上颌和下颌第一磨牙中观察到牙髓钙化。从唾液细胞中提取的基因组 DNA 被用于使用实时聚合酶链反应对八种基因多态性进行基因分型：EGF（rs2237051和rs4444903）、EGFR（rs2227983和rs763317）、TGFβ1（rs1800469和rs4803455）和TGFβR2（rs3087465和rs764522）。利用等位基因和基因型分布以及单倍型频率（P < 0.05）分析了牙髓钙化与基因多态性之间的关联。基因型分析和等位基因分布显示，所评估的生长因子与磨牙钙化之间没有统计学意义（P >0.05）。结论所调查的基因多态性与正畸患者的牙髓钙化无关。进一步的研究应调查编码生长因子的基因中的其他多态性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients

查看原文本刊更多论文

Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients

Background and objectives

Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), transforming growth factor-beta 1 (TGFβ1), and transforming growth factor-beta receptor 2 (TGFβR2) are associated with a risk of dental pulp calcifications in orthodontic patients.

Materials and methods

Digital orthopantomography (OPG) and genomic DNA from 132 patients were analyzed in this cross-sectional study. Pulp calcification was observed in the maxillary and mandibular first molars. Genomic DNA extracted from saliva cells was used to genotype eight genetic polymorphisms using real-time polymerase chain reaction: EGF (rs2237051 and rs4444903), EGFR (rs2227983 and rs763317), TGFβ1 (rs1800469 and rs4803455), and TGFβR2 (rs3087465 and rs764522). The association between pulp calcification and genetic polymorphisms was analyzed using allelic and genotypic distributions, and haplotype frequencies (P < 0.05).

Results

The prevalence of pulp calcification was 42.4 % in 490 molars. Genotypic analysis and allelic distribution showed no statistically significant association between the evaluated growth factors and molar calcification (P > 0.05). No haplotype combinations showed a statistically significant difference (P > 0.05).

Conclusion

The genetic polymorphisms investigated were not associated with dental pulp calcification in orthodontic patients. Further studies should investigate other polymorphisms in genes encoding growth factors.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.