Vladimir V. Botvin , Ekaterina V. Sukhinina , Anastasia A. Fetisova , Dmitry V. Wagner , Maria Y. Vedyashkina , Artyom Pryadko , Alexandra G. Pershina , Maria A. Surmeneva , Andrei L. Kholkin , Roman A. Surmenev
{"title":"交变磁场刺激下高掺杂 Fe3O4-CA 纳米颗粒的 P(VDF-TrFE)支架上正常人成纤维细胞基因表达谱的变化","authors":"Vladimir V. Botvin , Ekaterina V. Sukhinina , Anastasia A. Fetisova , Dmitry V. Wagner , Maria Y. Vedyashkina , Artyom Pryadko , Alexandra G. Pershina , Maria A. Surmeneva , Andrei L. Kholkin , Roman A. Surmenev","doi":"10.1016/j.eurpolymj.2024.113492","DOIUrl":null,"url":null,"abstract":"<div><div>The design of novel hybrid magnetoactive scaffolds based on biocompatible piezopolymers and magnetic nanoparticles is of interest for medicine, mainly for tissue regeneration, because application of an external either static or alternating magnetic field to cells that settled on a magnetoactive scaffold offers an opportunity for remote control of cellular functions. This study describes fabrication of electrospun magnetoactive poly(vinylidene fluoride-<em>co</em>-trifluoroethylene) [P(VDF-TrFE)] scaffolds highly doped with 20 wt% of magnetite nanoparticles modified with citric acid (Fe<sub>3</sub>O<sub>4</sub>-CA). The electrospun P(VDF-TrFE)/Fe<sub>3</sub>O<sub>4</sub>-CA scaffolds have defect-free morphology with a fiber diameter of approximately 1 μm and contain both an electroactive β-phase (predominantly) and a lesser amount of an γ-phase. A high content of uniformly distributed Fe<sub>3</sub>O<sub>4</sub>-CA nanoparticles within P(VDF-TrFE) fibrous scaffolds resulted in a high saturation magnetization of 12.1 emu/g and ferrimagnetic behavior of the composite P(VDF-TrFE)/Fe<sub>3</sub>O<sub>4</sub>-CA scaffolds. They were proved to be biocompatible with normal human cells: normal human fibroblasts and human mesenchymal stem cells adhered to the scaffold and retained their viability. According to high-throughput RNA-sequencing data, the adhesion of fibroblasts to the scaffolds upregulated genes related to key stages of tissue regeneration such as coagulation (genes <em>THBD</em> and <em>SERPINB2</em>) and wound healing (<em>IL24</em>, <em>PDGFB</em>, <em>F3</em>, and <em>PLAU</em>) and affected TGFβ, BMP, and Wnt signaling pathways. Alternating-magnetic-field exposure of the magnetoactive P(VDF-TrFE)/Fe<sub>3</sub>O<sub>4</sub>-CA scaffolds with fibroblasts settled on the surface activated extracellular and intracellular cell signaling pathways.</div></div>","PeriodicalId":315,"journal":{"name":"European Polymer Journal","volume":"220 ","pages":"Article 113492"},"PeriodicalIF":5.8000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Changes in gene expression profile of normal human fibroblasts on P(VDF-TrFE) scaffolds highly doped with Fe3O4-CA nanoparticles under alternating magnetic field stimulation\",\"authors\":\"Vladimir V. Botvin , Ekaterina V. Sukhinina , Anastasia A. Fetisova , Dmitry V. Wagner , Maria Y. Vedyashkina , Artyom Pryadko , Alexandra G. Pershina , Maria A. Surmeneva , Andrei L. Kholkin , Roman A. Surmenev\",\"doi\":\"10.1016/j.eurpolymj.2024.113492\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The design of novel hybrid magnetoactive scaffolds based on biocompatible piezopolymers and magnetic nanoparticles is of interest for medicine, mainly for tissue regeneration, because application of an external either static or alternating magnetic field to cells that settled on a magnetoactive scaffold offers an opportunity for remote control of cellular functions. This study describes fabrication of electrospun magnetoactive poly(vinylidene fluoride-<em>co</em>-trifluoroethylene) [P(VDF-TrFE)] scaffolds highly doped with 20 wt% of magnetite nanoparticles modified with citric acid (Fe<sub>3</sub>O<sub>4</sub>-CA). The electrospun P(VDF-TrFE)/Fe<sub>3</sub>O<sub>4</sub>-CA scaffolds have defect-free morphology with a fiber diameter of approximately 1 μm and contain both an electroactive β-phase (predominantly) and a lesser amount of an γ-phase. A high content of uniformly distributed Fe<sub>3</sub>O<sub>4</sub>-CA nanoparticles within P(VDF-TrFE) fibrous scaffolds resulted in a high saturation magnetization of 12.1 emu/g and ferrimagnetic behavior of the composite P(VDF-TrFE)/Fe<sub>3</sub>O<sub>4</sub>-CA scaffolds. They were proved to be biocompatible with normal human cells: normal human fibroblasts and human mesenchymal stem cells adhered to the scaffold and retained their viability. According to high-throughput RNA-sequencing data, the adhesion of fibroblasts to the scaffolds upregulated genes related to key stages of tissue regeneration such as coagulation (genes <em>THBD</em> and <em>SERPINB2</em>) and wound healing (<em>IL24</em>, <em>PDGFB</em>, <em>F3</em>, and <em>PLAU</em>) and affected TGFβ, BMP, and Wnt signaling pathways. 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Changes in gene expression profile of normal human fibroblasts on P(VDF-TrFE) scaffolds highly doped with Fe3O4-CA nanoparticles under alternating magnetic field stimulation
The design of novel hybrid magnetoactive scaffolds based on biocompatible piezopolymers and magnetic nanoparticles is of interest for medicine, mainly for tissue regeneration, because application of an external either static or alternating magnetic field to cells that settled on a magnetoactive scaffold offers an opportunity for remote control of cellular functions. This study describes fabrication of electrospun magnetoactive poly(vinylidene fluoride-co-trifluoroethylene) [P(VDF-TrFE)] scaffolds highly doped with 20 wt% of magnetite nanoparticles modified with citric acid (Fe3O4-CA). The electrospun P(VDF-TrFE)/Fe3O4-CA scaffolds have defect-free morphology with a fiber diameter of approximately 1 μm and contain both an electroactive β-phase (predominantly) and a lesser amount of an γ-phase. A high content of uniformly distributed Fe3O4-CA nanoparticles within P(VDF-TrFE) fibrous scaffolds resulted in a high saturation magnetization of 12.1 emu/g and ferrimagnetic behavior of the composite P(VDF-TrFE)/Fe3O4-CA scaffolds. They were proved to be biocompatible with normal human cells: normal human fibroblasts and human mesenchymal stem cells adhered to the scaffold and retained their viability. According to high-throughput RNA-sequencing data, the adhesion of fibroblasts to the scaffolds upregulated genes related to key stages of tissue regeneration such as coagulation (genes THBD and SERPINB2) and wound healing (IL24, PDGFB, F3, and PLAU) and affected TGFβ, BMP, and Wnt signaling pathways. Alternating-magnetic-field exposure of the magnetoactive P(VDF-TrFE)/Fe3O4-CA scaffolds with fibroblasts settled on the surface activated extracellular and intracellular cell signaling pathways.
期刊介绍:
European Polymer Journal is dedicated to publishing work on fundamental and applied polymer chemistry and macromolecular materials. The journal covers all aspects of polymer synthesis, including polymerization mechanisms and chemical functional transformations, with a focus on novel polymers and the relationships between molecular structure and polymer properties. In addition, we welcome submissions on bio-based or renewable polymers, stimuli-responsive systems and polymer bio-hybrids. European Polymer Journal also publishes research on the biomedical application of polymers, including drug delivery and regenerative medicine. The main scope is covered but not limited to the following core research areas:
Polymer synthesis and functionalization
• Novel synthetic routes for polymerization, functional modification, controlled/living polymerization and precision polymers.
Stimuli-responsive polymers
• Including shape memory and self-healing polymers.
Supramolecular polymers and self-assembly
• Molecular recognition and higher order polymer structures.
Renewable and sustainable polymers
• Bio-based, biodegradable and anti-microbial polymers and polymeric bio-nanocomposites.
Polymers at interfaces and surfaces
• Chemistry and engineering of surfaces with biological relevance, including patterning, antifouling polymers and polymers for membrane applications.
Biomedical applications and nanomedicine
• Polymers for regenerative medicine, drug delivery molecular release and gene therapy
The scope of European Polymer Journal no longer includes Polymer Physics.