合成用于增强 SERS 检测的新型二氧化硅封装核心卫星纳米标签

IF 3.3 3区物理与天体物理 Q2 OPTICS

Journal of Luminescence Pub Date : 2024-10-10 DOI:10.1016/j.jlumin.2024.120935

Haoquan Wang , Qi Yang , Yan Zhang , Bo Peng , Feng Wu , Zewei Quan

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引用次数: 0

摘要

开发具有高信号强度、优异信号稳定性和生物相容性的 SERS 纳米标签在食品安全、药物检测和病毒检测领域具有重要意义。在这项工作中，均匀合成了二氧化硅包封、吲哚菁绿（ICG）修饰的金八面体核心-小金球形纳米颗粒（NPs）卫星纳米结构（Au/Au@ICG@SiO2），作为表面增强拉曼散射（SERS）标签，既提供了丰富的 "热点"，又增加了拉曼报告分子ICG的稳定性，为后续的功能化和检测提供了极大的便利。实验和数值结果表明，这种金/金@ICG 纳米结构对水溶液中 ICG 的检测限达到了 10-⁷ M，这归因于与金八面体 NPs 相比热点数量的增加。制备的金/金@ICG@SiO2 SERS 纳米标签进一步与横向流免疫测定（LFIA）相结合，用于检测 SARS-CoV-2 核头壳蛋白。与检出限为 8 pg/mL 的传统侧流免疫分析条上的生物识别方法相比，基于这种新型 SERS 标签和定制的自适应拉曼绘图光谱仪，SARS-CoV-2 核苷酸蛋白的检出能力提高了约 16 倍（500 fg/mL）。这种 Au/Au@ICG@SiO2 SERS 标签有望在未来类似传染性病毒的早期检测、预防和控制中发挥重要作用。

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Synthesis of novel silica-encapsulated core-satellite nanotags for enhanced SERS detection

Developing a SERS nanotag with high signal intensity, excellent signal stability, and biocompatibility is of significant importance in the fields of food safety, drug testing, and virus detection. In this work, silica-encapsulated, indocyanine green (ICG)-modified Au octahedral core-small Au spherical nanoparticles (NPs) satellite nanostructures (Au/Au@ICG@SiO₂) are uniformly synthesized as surface-enhanced Raman scattering (SERS) tags, which provide abundant “hot spots” and also increase the stability of Raman reporter molecule ICG to greatly facilitate subsequent functionalization and detection. Both experimental and numerical results demonstrate that this Au/Au@ICG nanostructure achieve a detection limit of 10⁻⁷ M for ICG in aqueous solution ascribed to the increased number of hot spots compared with Au octahedral NPs. As-prepared Au/Au@ICG@SiO₂ SERS nanotags are further integrated with a lateral flow immunoassay (LFIA) for the detection of the SARS-CoV-2 nucleocapsid protein. Compared to the traditional bio-recognition method on LFIA strips with the detection limit of 8 pg/mL, the detection capability for the SARS-CoV-2 nucleocapsid protein is improved approximately 16 times (500 fg/mL) based on this novel SERS tag and custom-built adaptive Raman mapping spectrometer. This Au/Au@ICG@SiO₂ SERS tag is expected to play a significant role in early detection, prevention, and control of similar infectious viruses in the future.

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来源期刊

Journal of Luminescence 物理-光学

CiteScore

6.70

自引率

13.90%

发文量

850

审稿时长

3.8 months

期刊介绍： The purpose of the Journal of Luminescence is to provide a means of communication between scientists in different disciplines who share a common interest in the electronic excited states of molecular, ionic and covalent systems, whether crystalline, amorphous, or liquid. We invite original papers and reviews on such subjects as: exciton and polariton dynamics, dynamics of localized excited states, energy and charge transport in ordered and disordered systems, radiative and non-radiative recombination, relaxation processes, vibronic interactions in electronic excited states, photochemistry in condensed systems, excited state resonance, double resonance, spin dynamics, selective excitation spectroscopy, hole burning, coherent processes in excited states, (e.g. coherent optical transients, photon echoes, transient gratings), multiphoton processes, optical bistability, photochromism, and new techniques for the study of excited states. This list is not intended to be exhaustive. Papers in the traditional areas of optical spectroscopy (absorption, MCD, luminescence, Raman scattering) are welcome. Papers on applications (phosphors, scintillators, electro- and cathodo-luminescence, radiography, bioimaging, solar energy, energy conversion, etc.) are also welcome if they present results of scientific, rather than only technological interest. However, papers containing purely theoretical results, not related to phenomena in the excited states, as well as papers using luminescence spectroscopy to perform routine analytical chemistry or biochemistry procedures, are outside the scope of the journal. Some exceptions will be possible at the discretion of the editors.