用于生物成像的具有强单、双光子激发荧光的小分子探针

IF 3.3 3区物理与天体物理 Q2 OPTICS

Journal of Luminescence Pub Date : 2024-09-30 DOI:10.1016/j.jlumin.2024.120921

Jinghua Zhang , Yue Tang , Qichuan Duan , Yaling Wang , Xiaoqiang Tong , Dongliang Tao , Rongbao Liao , Feng Jin , Kun Yin , Deqian Huang , Shangrong Zhang

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引用次数: 0

摘要

双光子激发荧光（TPEF）因其卓越的深层组织穿透性和出色的高时空分辨率而在生物成像应用中广受欢迎。在本文中，苯并噻唑和苯并咪唑通过吡啶基分别与 N，N-二苯基苯胺相连。设计并合成了两种新的 D-π-A（电子供体-π共轭桥-电子受体）探针（P1、P2）。通过傅立叶变换红外光谱（FT-IR）、1H NMR、13C NMR、ESI-MS 和 X 射线单晶衍射对分子结构进行了完整的表征。探针具有良好的单光子激发荧光和双光子激发荧光。实验测试结果表明，这些探针可用于 HePG2 和幼年斑马鱼的生物成像。此外，该荧光探针还具有靶向定位癌细胞线粒体的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Small molecule probes with strong one and two-photon excited fluorescence for bioimaging

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Small molecule probes with strong one and two-photon excited fluorescence for bioimaging

Two-photon excited fluorescence (TPEF) is widely sought after in bioimaging applications due to its exceptional deep tissue penetration and excellent high spatiotemporal resolution. In this paper, benzothiazole and benzimidazole are linked to N, N-diphenylaniline, respectively, through pyridine vinyl. Two new D-π-A (electron donor-π conjugated bridges-electron acceptor) probes (P1, P2) were designed and synthesized. The molecular structures were completely characterized through FT-IR, ¹H NMR, ¹³C NMR, ESI-MS, and X-ray single crystal diffraction. The probes have good one-photon excited fluorescence and two-photon excited fluorescence. Experimental test results indicate that they can be fluorescence probes for biological imaging of HePG2 and juvenile zebrafish. In addition, the fluorescent probes also have the ability to target and locate cancer cell mitochondria.

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来源期刊

Journal of Luminescence 物理-光学

CiteScore

6.70

自引率

13.90%

发文量

850

审稿时长

3.8 months

期刊介绍： The purpose of the Journal of Luminescence is to provide a means of communication between scientists in different disciplines who share a common interest in the electronic excited states of molecular, ionic and covalent systems, whether crystalline, amorphous, or liquid. We invite original papers and reviews on such subjects as: exciton and polariton dynamics, dynamics of localized excited states, energy and charge transport in ordered and disordered systems, radiative and non-radiative recombination, relaxation processes, vibronic interactions in electronic excited states, photochemistry in condensed systems, excited state resonance, double resonance, spin dynamics, selective excitation spectroscopy, hole burning, coherent processes in excited states, (e.g. coherent optical transients, photon echoes, transient gratings), multiphoton processes, optical bistability, photochromism, and new techniques for the study of excited states. This list is not intended to be exhaustive. Papers in the traditional areas of optical spectroscopy (absorption, MCD, luminescence, Raman scattering) are welcome. Papers on applications (phosphors, scintillators, electro- and cathodo-luminescence, radiography, bioimaging, solar energy, energy conversion, etc.) are also welcome if they present results of scientific, rather than only technological interest. However, papers containing purely theoretical results, not related to phenomena in the excited states, as well as papers using luminescence spectroscopy to perform routine analytical chemistry or biochemistry procedures, are outside the scope of the journal. Some exceptions will be possible at the discretion of the editors.